Biophysical characterization of the calmodulin-like domain of Plasmodium falciparum calcium dependent protein kinase 3

Funding Agencies|Vetenskapsradet [Dnr. 2012-5136, Dnr. 2012-5270]; Swedish e-science
Calcium dependent protein kinases are unique to plants and certain parasites and comprise an N-terminal segment and a kinase domain that is regulated by a C-terminal calcium binding domain. Since the proteins are not found in man they are potential drug targets. We have characterized the calcium binding lobes of the regulatory domain of calcium dependent protein kinase 3 from the malaria parasite Plasmodium falciparum. Despite being structurally similar, the two lobes differ in several other regards. While the monomeric N-terminal lobe changes its structure in response to calcium binding and shows global dynamics on the sub-millisecond time-scale both in its apo and calcium bound states, the C-terminal lobe could not be prepared calcium-free and forms dimers in solution. If our results can be generalized to the full-length protein, they suggest that the C-terminal lobe is calcium bound even at basal levels and that activation is caused by the structural reorganization associated with binding of a single calcium ion to the N-terminal lobe.

Niklasson, MarkusLinköpings universitet,Kemi,Tekniska fakulteten(Swepub:liu)marni23 (author)
Cassman Eklöf, SofieLinköpings universitet,Kemi,Tekniska fakulteten (author)
Wallner, BjörnLinköpings universitet,Bioinformatik,Tekniska fakulteten(Swepub:liu)bjowa51 (author)
Lundström, PatrikLinköpings universitet,Kemi,Tekniska fakulteten(Swepub:liu)patlu71 (author)
Linköpings universitetKemi (creator_code:org_t)

By the author/editor: Andrésen, Cecili ...; Niklasson, Marku ...; Cassman Eklöf, S ...; Wallner, Björn; Lundström, Patri ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Structural Biolo ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.