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HIVIS-DNA or HIVISo...
HIVIS-DNA or HIVISopt-DNA priming followed by CMDR vaccinia-based boosts induce both humoral and cellular murine immune responses to HIV.
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- Hinkula, Jorma (författare)
- Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten,Department of Microbiology Tumor and Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden
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- Petkov, S (författare)
- Karolinska Institutet
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- Ljungberg, K (författare)
- Karolinska Institutet
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- Hallengärd, D (författare)
- Department of Microbiology Tumor and Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden
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- Bråve, A (författare)
- Department of Microbiology Tumor and Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden
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- Isaguliants, M (författare)
- Karolinska Institutet
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- Falkeborn, Tina (författare)
- Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten
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- Sharma, Sumit (författare)
- Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten
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- Liakina, V (författare)
- Faculty of Medicine, Vilnius University 2, 08661 Vilnius, Lithuania
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- Robb, M (författare)
- U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, 20892 MD, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, 20892 MD, USA
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- Eller, M (författare)
- U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, 20892 MD, USA / Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, 20892 MD, USA
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- Moss, B (författare)
- Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, 20892 MD, USA
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- Biberfeld, G (författare)
- Karolinska Institutet
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- Sandström, E (författare)
- Department of South Hospital, Karolinska Institutet, 11883 Stockholm, Sweden
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- Nilsson, C (författare)
- Karolinska Institutet
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- Markland, K (författare)
- Clinical Research Center and Vecura, Karolinska University Hospital, 17176 Stockholm, Sweden
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- Blomberg, P (författare)
- Karolinska Institutet
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- Wahren, B (författare)
- Karolinska Institutet
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(creator_code:org_t)
- Elsevier, 2017
- 2017
- Engelska.
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Ingår i: Heliyon. - : Elsevier. - 2405-8440. ; 3:6
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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http://www.cell.com/...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- BACKGROUND: In order to develop a more effective prophylactic HIV-1 vaccine it is important optimize the components, improve Envelope glycoprotein immunogenicity as well as to explore prime-boost immunization schedules. It is also valuable to include several HIV-1 subtype antigens representing the world-wide epidemic.METHODS: HIVIS-DNA plasmids which include Env genes of subtypes A, B and C together with Gag subtypes A and B and RTmut/Rev of subtype B were modified as follows: the Envelope sequences were shortened, codon optimized, provided with an FT4 sequence and an immunodominant region mutated. The reverse transcriptase (RT) gene was shortened to contain the most immunogenic N-terminal fragment and fused with an inactivated viral protease vPR gene. HIVISopt-DNA thus contains fewer plasmids but additional PR epitopes compared to the native HIVIS-DNA. DNA components were delivered intradermally to young Balb/c mice once, using a needle-free Biojector® immediately followed by dermal electroporation. Vaccinia-based MVA-CMDR boosts including Env gene E and Gag-RT genes A were delivered intramuscularly by needle, once or twice.RESULTS: Both HIVIS-DNA and HIVISopt-DNA primed humoral and cell mediated responses well. When boosted with heterologous MVA-CMDR (subtypes A and E) virus inhibitory neutralizing antibodies were obtained to HIV-1 subtypes A, B, C and AE. Both plasmid compositions boosted with MVA-CMDR generated HIV-1 specific cellular responses directed against HIV-1 Env, Gag and Pol, as measured by IFNγ ELISpot. It was shown that DNA priming augmented the vector MVA immunological boosting effects, the HIVISopt-DNA with a trend to improved (Env) neutralization, the HIVIS-DNA with a trend to better (Gag) cell mediated immune reponses.CONCLUSIONS: HIVIS-DNA was modified to obtain HIVISopt-DNA that had fewer plasmids, and additional epitopes. Even with one DNA prime followed by two MVA-CMDR boosts, humoral and cell-mediated immune responses were readily induced by priming with either DNA construct composition. Priming by HIV-DNA augmented neutralizing antibody responses revealed by boosting with the vaccinia-based heterologous sequences. Cellular and antibody responses covered selected strains representing HIV-1 subtypes A, B, C and CRF01_AE. We assume this is related to the inclusion of heterologous full genes in the vaccine schedule.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- Immunology
- Infectious disease
- Vaccines
- Virology
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Hinkula, Jorma
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Petkov, S
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Ljungberg, K
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Hallengärd, D
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Bråve, A
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Isaguliants, M
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visa fler...
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Falkeborn, Tina
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Sharma, Sumit
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Liakina, V
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Robb, M
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Eller, M
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Moss, B
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Biberfeld, G
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Sandström, E
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Nilsson, C
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Markland, K
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Blomberg, P
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Wahren, B
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