Flow cytometry in Spermatology: A bright future ahead

Funding Agencies|Secretaria de Estado de Investigacion, Desarrollo e Innovacion [AGL2013-43211-R, IJCI-2014-21671]; Ministerio de Economia y Competitividad-FEDER, Madrid, Spain [AGL2013-43211-R]; Junta de Extremadura-FEDER [GR 10010, IB 16030]; Swedish Research Councils VR [521-2011-6353]; Formas [221-2011-512]; Ministerio de Educacion, Cultura y Deporte [IJCI-2014-21671]
Techniques such as mass spectrometry have led to unprecedented knowledge of the proteins that are present in the spermatozoa of humans and other mammals. However, in spite of their high-throughput and fractioning techniques, most of the techniques in use only offer average values for the entire sperm population. Yet, ejaculate is very heterogeneous, and average values may mask relevant biological information. The application of flow cytometry may overcome this disadvantage, allowing proteomic analysis at the single-cell level. Moreover, recent advances in cytometry, allowing multiple analyses within a single cell combined with powerful statistical tools, as an expanding subfield in spermatology, are described. The increased use of advanced flow cytometers in andrology laboratories will allow the rapid development of multiparametric, multicolour flow cytometry in andrology that will expand the clinical applications and research possibilities of flow cytometry-based proteomic approaches, especially in the subfields of clinical andrology and sperm biotechnology.

Gil, M. C.University of Extremadura, Spain (author)
Rodriguez-Martinez, HeribertoLinköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten(Swepub:liu)herro55 (author)
Anel, L.University of Leon, Spain (author)
Pena, F. J.University of Extremadura, Spain (author)
Martin-Munoz, P.University of Extremadura, Spain (author)
University of Leon, SpainUniversity of Extremadura, Spain (creator_code:org_t)

By the author/editor: Ortega-Ferrusola ...; Gil, M. C.; Rodriguez-Martin ...; Anel, L.; Pena, F. J.; Martin-Munoz, P.

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Biomedical Labor ...

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