Activation-induced FOXP3 isoform profile in peripheral CD4+T cells is associated with coronary artery disease

Lundberg, AnnaLinköpings universitet,Avdelningen för kardiovaskulär medicin,Medicinska fakulteten (author)

Funding Agencies|Swedish Research Council [349-2007-8703]; Swedish Heart-Lung Foundation; Foundation for Strategic Research (SSF); Vinnova Foundation; Stockholm County Council; European Commission (project Athero-Flux); European Commission (project VIA); [06816]
Background and aims: The expression of FOXP3 isoforms affects regulatory T (Treg) cell function. Reduced Treg cell function has been associated with coronary artery disease (CAD). However, alternative splicing of FOXP3 in CAD has not been investigated. Methods: FOXP3 splice variants and IL17A transcripts in peripheral blood mononuclear cells from stable CAD patients and healthy controls were quantified, and FOXP3 isoform expression in response to T cell receptor (TCR) stimulation or LDL was analyzed by flow cytometry. Results: Compared to healthy controls, CAD patients expressed significantly more FOXP3 transcripts that included exon 2, whereas alternative splicing of exon 7 in correlation with IL17A expression was reduced. Moreover, TCR stimulation, as well as exposure to LDL, decreased alternative splicing of FOXP3 in CD4+ T cells in vitro. Conclusions: Our results demonstrate that blood mononuclear cells in stable CAD patients express a ratio of FOXP3 isoforms that is characteristic for activated CD4+ T cells. (C) 2017 Elsevier B.V. All rights reserved.

Jonasson, LenaLinköpings universitet,Avdelningen för kardiovaskulär medicin,Medicinska fakulteten,Region Östergötland, Kardiologiska kliniken US(Swepub:liu)lenjo59 (author)
Hansson, Göran K.Karolinska Institutet,Karolinska University Hospital, Sweden (author)
Mailer, Reiner K. W.Karolinska University Hospital, Sweden (author)
Linköpings universitetAvdelningen för kardiovaskulär medicin (creator_code:org_t)

By the author/editor: Lundberg, Anna; Jonasson, Lena; Hansson, Göran K ...; Mailer, Reiner K ...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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