SwePub
Sök i LIBRIS databas

  Extended search

WFRF:(Azevedo Joana)
 

Search: WFRF:(Azevedo Joana) > (2017) > Cytotoxicity of por...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Ribeiro, TiagoCIIMAR, Portugal (author)

Cytotoxicity of portoamides in human cancer cells and analysis of the molecular mechanisms of action

  • Article/chapterEnglish2017

Publisher, publication year, extent ...

  • 2017-12-07
  • PUBLIC LIBRARY SCIENCE,2017
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:liu-143908
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-143908URI
  • https://doi.org/10.1371/journal.pone.0188817DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:137232854URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Funding Agencies|Structured Program of R&D&I INNOVMAR - Innovation and Sustainability in the Management and Exploitation of Marine Resources - Northern Regional Operational Program (NORTE) through the European Regional Development Fund (ERDF) [NORTE-01-0145-FEDER-000035]; national funds (FCT, Foundation for Science and Technology) [UID/Multi/04423/2013, UID/BIM/04501/2013, UID/IC/00051/2013]; RNEM (National Mass Spectrometry Network); FCT [IF/01358/2014, SFRH/BPD/112287/2015]
  • Portoamides are cyclic peptides produced and released by the cyanobacterial strain Phormidium sp. presumably to interfere with other organisms in their ecosystems (" allelopathy"). Portoamides were previously demonstrated to have an antiproliferative effect on human lung carcinoma cells, but the underlying mechanism of this activity has not been described. In the present work, the effects of portoamides on proliferation were examined in eight human cancer cell lines and two non-carcinogenic cell lines, and major differences in sensitivities were observed. To generate hypotheses with regard to molecular mechanisms of action, quantitative proteomics using 2D gel electrophoresis and MALDI-TOF/ TOF were performed on the colon carcinoma cell line HT-29. The expression of proteins involved in energy metabolism (mitochondrial respiratory chain and pentose phosphate pathway) was found to be affected. The hypothesis of altered energy metabolism was tested in further experiments. Exposure to portoamides resulted in reduced cellular ATP content, likely due to decreased mitochondrial energy production. Mitochondrial hyperpolarization and reduced mitochondrial reductive capacity was observed in treated cells. Furthermore, alterations in the expression of peroxiredoxins (PRDX4, PRDX6) and components of proteasome subunits (PSB4, PSA6) were observed in portoamide-treated cells, but these alterations were not associated with detectable increases in oxidative stress. We conclude that the cytotoxic activity of portoamides is associated with disturbance of energy metabolism, and alterations in mitochondrial structure and function.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Lemos, FilipaCIIMAR, Portugal (author)
  • Preto, MarcoCIIMAR, Portugal (author)
  • Azevedo, JoanaCIIMAR, Portugal (author)
  • Ligla Sousa, MariaCIIMAR, Portugal (author)
  • Leao, Pedro N.CIIMAR, Portugal (author)
  • Campos, AlexandreCIIMAR, Portugal (author)
  • Linder, StigKarolinska Institutet,Linköpings universitet,Avdelningen för läkemedelsforskning,Medicinska fakulteten,Karolinska Institute, Sweden(Swepub:liu)stili13 (author)
  • Vitorino, RuiUniversity of Aveiro, Portugal; University of Porto, Portugal (author)
  • Vasconcelos, VitorCIIMAR, Portugal; University of Porto, Portugal (author)
  • Urbatzka, RalphCIIMAR, Portugal (author)
  • CIIMAR, PortugalAvdelningen för läkemedelsforskning (creator_code:org_t)

Related titles

  • In:PLOS ONE: PUBLIC LIBRARY SCIENCE12:121932-6203

Internet link

Find in a library

  • PLOS ONE (Search for host publication in LIBRIS)

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view