An inter-laboratory comparison study on transfer, persistence and recovery of DNA from cable ties

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Steensma, KristyNetherlands Forens Institute, Netherlands (author)

An inter-laboratory comparison study on transfer, persistence and recovery of DNA from cable ties

Article/chapterEnglish2017

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ELSEVIER IRELAND LTD,2017
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LIBRIS-ID:oai:DiVA.org:liu-143998
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-143998URI
https://doi.org/10.1016/j.fsigen.2017.08.015DOI

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Language:English
Summary in:English

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Funding Agencies|European Commission through European Network of Forensic Science Institutes (ENFSI) [MP2013 T5]; Prevention of and Fight against Crime Programme of the European Union [HOME/2013/ISEC/MO/ENFSI/4000005962]
To address questions on the activity that led to the deposition of biological traces in a particular case, general information on the probabilities of transfer, persistence and recovery of cellular material in relevant scenarios is necessary. These figures may be derived from experimental data described in forensic literature when conditions relevant to the case were included. The experimental methodology regarding sampling, DNA extraction, DNA typing and profile interpretation that were used to generate these published data may differ from those applied in the case and thus the applicability of the literature data may be questioned. To assess the level of variability that different laboratories obtain when similar exhibits are analysed, we performed an inter-laboratory study between four partner laboratories. Five sets of 20 cable ties bound by different volunteers were distributed to the participating laboratories and sampled and processed according to the in-house protocols. Differences were found for the amount of retrieved DNA, as well as for the reportability and composition of the DNA profiles. These differences also resulted in different probabilities of transfer, persistence and recovery for each laboratory. Nevertheless, when applied to a case example, these differences resulted in similar assignments of weight of evidence given activity-level propositions.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Medicinsk genetik hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Medical Genetics hsv//eng
Forensic science; DNA transfer; DNA persistence; Tie-wrap; Cable tie; Criminalistics; Activity-level reporting

Added entries (persons, corporate bodies, meetings, titles ...)

Ansell, RickyLinköpings universitet,Biologi,Tekniska fakulteten,Swedish National Forens Centre, SE-58194 Linkoping, Sweden(Swepub:liu)rican78 (author)
Clarisse, LindyNetherlands Forens Institute, Netherlands (author)
Connolly, EdwardForens Science Ireland, Ireland (author)
Kloosterman, Ate D.Netherlands Forens Institute, Netherlands; University of Amsterdam, Netherlands (author)
McKenna, Louise G.Forens Science Ireland, Ireland (author)
van Oorschot, Roland A. H.Victoria Police Forens Serv Department, Australia (author)
Szkuta, BiancaVictoria Police Forens Serv Department, Australia; Deakin University, Australia (author)
Kokshoorn, BasNetherlands Forens Institute, Netherlands (author)
Netherlands Forens Institute, NetherlandsBiologi (creator_code:org_t)

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In:Forensic Science International: ELSEVIER IRELAND LTD31, s. 95-1041872-49731878-0326

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By the author/editor: Steensma, Kristy; Ansell, Ricky; Clarisse, Lindy; Connolly, Edward; Kloosterman, Ate ...; McKenna, Louise ...; show more...; van Oorschot, Ro ...; Szkuta, Bianca; Kokshoorn, Bas; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Medical Genetics

Articles in the publication: Forensic Science ...

By the university: Linköping University

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