Sökning: WFRF:(Sundqvist Per)
> (2000-2004) >
Inducible nitric ox...
Inducible nitric oxide synthase (NOS II) is constitutive in human neutrophils
-
- Cedergren, Jan (författare)
- Linköpings universitet,Reumatologi,Hälsouniversitetet
-
- Follin, Per (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Infektionsmedicin,Hälsouniversitetet,Infektionskliniken i Östergötland
-
- Forslund, Tony (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
-
visa fler...
-
- Lindmark, Maria (författare)
- Linköpings universitet,Institutionen för medicin och vård,Hälsouniversitetet
-
- Sundqvist, Tommy (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
-
- Skogh, Thomas (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Reumatologi,Hälsouniversitetet,Länskliniken för Reumatologi i Östergötland
-
visa färre...
-
(creator_code:org_t)
- Wiley, 2003
- 2003
- Engelska.
-
Ingår i: APMIS. - : Wiley. - 0903-4641 .- 1600-0463. ; 111:10, s. 963-968
- Relaterad länk:
-
http://urn.kb.se/res...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The objective was to study the expression of inducible nitric oxide synthase (NOS II) in and NO production by human blood neutrophils and in in vivo exudated neutrophils. Cellular expression of NOS II was evaluated by flow cytometry in whole blood, in isolated blood neutrophils, and in neutrophils obtained by exudation in vivo into skin chambers. Neutrophil NOS II was also demonstrated by Western blotting. Uptake of 3H-labelled L-arginine was studied in vitro and NOS activity measured in a whole cell assay by the conversion of 3H-arginine to 3H-citrulline. In contrast to unseparated blood cells, NOS II was demonstrable both in isolated blood neutrophils and exudated cells. The failure to detect NOS II by flow cytometry in whole blood cells thus proved to be due to the quenching effect of hemoglobin. Western blotting revealed a 130 kD band corresponding to NOS II in isolated blood neutrophils, but detection was dependent on diisopropylfluorophosphate for proteinase inhibition. L-arginine was taken up by neutrophils, but enzymatic activity could not be demonstrated. We conclude that human neutrophils constitutively express NOS II, but that its demonstration by FITC-labelling is inhibited by hemoglobin-mediated quenching in whole blood samples.
Nyckelord
- Inflammation
- nitric oxide
- iNOS
- granulocytes
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
APMIS
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas