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Labeling nanopartic...
Labeling nanoparticles : Dye leakage and altered cellular uptake
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- Snipstad, Sofie (författare)
- Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway
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- Hak, Sjoerd (författare)
- Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway; Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway
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- Baghirov, Habib (författare)
- Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway
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- Sulheim, Einar (författare)
- Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway; SINTEF Materials and Chemistry, Trondheim, Norway
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- Mørch, Ýrr (författare)
- SINTEF Materials and Chemistry, Trondheim, Norway
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- Lélu, Sylvie (författare)
- Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway
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- von Haartman, Eva (författare)
- Pharmaceutical Sciences Laboratory, Åbo Akademi University, Turku, Finland; Laboratory of Physical Chemistry, Åbo Akademi University, Turku, Finland
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- Bäck, Marcus, 1979- (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Nilsson, K. Peter R., 1970- (författare)
- Linköpings universitet,Kemi,Tekniska fakulteten
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- Klymchenko, Andrey S (författare)
- Laboratoire de Biophotonique et Pharmacologie, UMR CNRS 7213, Université de Strasbourg, Strasbourg, France
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- de Lange Davies, Catharina (författare)
- Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway
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- Åslund, Andreas K. O. (författare)
- Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway
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(creator_code:org_t)
- 2016-04-14
- 2017
- Engelska.
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Ingår i: Cytometry Part A. - : John Wiley & Sons. - 1552-4922 .- 1552-4930. ; 91:8, s. 760-766
- Relaterad länk:
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https://onlinelibrar...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In vitro and in vivo behavior of nanoparticles (NPs) is often studied by tracing the NPs with fluorescent dyes. This requires stable incorporation of dyes within the NPs, as dye leakage may give a wrong interpretation of NP biodistribution, cellular uptake, and intracellular distribution. Furthermore, NP labeling with trace amounts of dye should not alter NP properties such as interactions with cells or tissues. To allow for versatile NP studies with a variety of fluorescence-based assays, labeling of NPs with different dyes is desirable. Hence, when new dyes are introduced, simple and fast screening methods to assess labeling stability and NP-cell interactions are needed. For this purpose, we have used a previously described generic flow cytometry assay; incubation of cells with NPs at 4 and 37C. Cell-NP interaction is confirmed by cellular fluorescence after 37C incubation, and NP-dye retention is confirmed when no cellular fluorescence is detected at 4C. Three different NP-platforms labeled with six different dyes were screened, and a great variability in dye retention was observed. Surprisingly, incorporation of trace amounts of certain dyes was found to reduce or even inhibit NP uptake. This work highlights the importance of thoroughly evaluating every dye-NP combination before pursuing NP-based applications. © 2016 International Society for Advancement of Cytometry.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- cellular uptake; flow cytometry; leakage; liposomes; nanoemulsions; polymeric nanoparticles
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Snipstad, Sofie
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Hak, Sjoerd
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Baghirov, Habib
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Sulheim, Einar
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Mørch, Ýrr
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Lélu, Sylvie
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visa fler...
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von Haartman, Ev ...
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Bäck, Marcus, 19 ...
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Nilsson, K. Pete ...
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Klymchenko, Andr ...
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de Lange Davies, ...
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Åslund, Andreas ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Cellbiologi
- Artiklar i publikationen
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Cytometry Part A
- Av lärosätet
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Linköpings universitet