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An electrochemical ...
An electrochemical immunosensor for cardiac Troponin I using electrospun carboxylated multi-walled carbon nanotube-whiskered nanofibres
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- Rezaei, Babak (author)
- Nanotechnology Institute, Amirkabir University of Technology, Tehran, Iran
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- Shoushtari, Ahmad Mousavi (author)
- Textile Engineering Department, AmirKabir University of Technology, Tehran, Iran
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- Rabiee, Mohammad (author)
- Biomaterials Group, Biomedical Engineering Department, Amirkabir University of Technology, Tehran, Iran
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- Uzun, Lokman, 1978- (author)
- Linköpings universitet,Sensor- och aktuatorsystem,Tekniska fakulteten
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- Wing Cheung, Mak, 1977- (author)
- Linköpings universitet,Sensor- och aktuatorsystem,Tekniska fakulteten
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- TURNER, APF, 1950- (author)
- Linköpings universitet,Sensor- och aktuatorsystem,Tekniska fakulteten
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(creator_code:org_t)
- Elsevier, 2018
- 2018
- English.
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In: Talanta. - : Elsevier. - 0039-9140 .- 1873-3573. ; 182, s. 178-186
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- A sandwich-type nanostructured immunosensor based on carboxylated multi-walled carbon nanotube (CMWCNT)-embedded whiskered nanofibres (WNFs) was developed for detection of cardiac Troponin I (cTnI). WNFs were directly fabricated on glassy carbon electrodes (GCE) by removing the sacrificial component (polyethylene glycol, PEG) after electrospinning of polystyrene/CMWCNT/PEG nanocomposite nanofibres, and utilised as a transducer layer for enzyme-labeled amperometric immunoassay of cTnI. The whiskered segments of CMWCNTs were activated and utilised to immobilise anti-cTnT antibodies. It was observed that the anchored CMWCNTs within the nanofibres were suitably stabilised with excellent electrochemical repeatability. A sandwich-type immuno-complex was formed between cTnI and horseradish peroxidase-conjugated anti-cTnI (HRP-anti-cTnI). The amperometric responses of the immunosensor were studied using cyclic voltammetry (CV) through an enzymatic reaction between hydrogen peroxide and HRP conjugated to the secondary antibody. The nanostructured immunosensor delivered a wide detection range for cTnI from the clinical borderline for a normal person (0.5-2 ng mL(-1)) to the concentration present in myocardial infarction patients (amp;gt; 20 ng mL(-1)), with a detection limit of similar to 0.04 ng mL(-1). It also showed good reproducibility and repeatability for three different cTnI concentration (1, 10 and 25 ng mL(-1)) with satisfactory relative standard deviations (RSD). Hence, the proposed nanostructured immunosensor shows potential for point-of-care testing.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- Immunosensor; Cardiac Troponin I; Carbon nanotubes; Electrospinning; Whiskered nanofibres
Publication and Content Type
- ref (subject category)
- art (subject category)
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