Sökning: onr:"swepub:oai:DiVA.org:liu-148383" >
Codon optimization ...
-
Latanova, A. A.Russian Acad Sci, Russia; Karolinska Inst, Sweden; Gamaleja Res Ctr Epidemiol and Microbiol, Russia
(författare)
Codon optimization and improved delivery/immunization regimen enhance the immune response against wild-type and drug-resistant HIV-1 reverse transcriptase, preserving its Th2-polarity
- Artikel/kapitelEngelska2018
Förlag, utgivningsår, omfång ...
-
2018-05-24
-
NATURE PUBLISHING GROUP,2018
-
electronicrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:liu-148383
-
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-148383URI
-
https://doi.org/10.1038/s41598-018-26281-zDOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:138366263URI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Funding Agencies|Russian Fund for Basic Research [17-54-30002]; RAS Presidium Program "Molecular and cell biology and post-genome technologies" [01201456591]; Russian Science Foundation [15-15-30039]; EU Twinning project VACTRAIN [692293]; PI project of the Swedish Institute [19806_2016 INNOVIMMUNE]; VACTRAIN [692293]; INNOVIMMUNE; Ministry of Education and Science of the Russian Federation [K2-2016-069]
-
DNA vaccines require a considerable enhancement of immunogenicity. Here, we optimized a prototype DNA vaccine against drug-resistant HIV-1 based on a weak Th2-immunogen, HIV-1 reverse transcriptase (RT). We designed expression-optimized genes encoding inactivated wild-type and drug-resistant RTs (RT-DNAs) and introduced them into mice by intradermal injections followed by electroporation. RT-DNAs were administered as single or double primes with or without cyclic-di-GMP, or as a prime followed by boost with RT-DNA mixed with a luciferase-encoding plasmid ("surrogate challenge"). Repeated primes improved cellular responses and broadened epitope specificity. Addition of cyclic-di-GMP induced a transient increase in IFN-gamma production. The strongest anti-RT immune response was achieved in a prime-boost protocol with electroporation by short 100V pulses done using penetrating electrodes. The RT-specific response, dominated by CD4+T-cells, targeted epitopes at aa 199-220 and aa 528-543. Drug-resistance mutations disrupted the epitope at aa 205-220, while the CTL epitope at aa 202-210 was not affected. Overall, multiparametric optimization of RT strengthened its Th2- performance. A rapid loss of RT/luciferase-expressing cells in the surrogate challenge experiment revealed a lytic potential of anti-RT response. Such lytic CD4+ response would be beneficial for an HIV vaccine due to its comparative insensitivity to immune escape.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Petkov, S.Karolinska Institutet,Karolinska Inst, Sweden
(författare)
-
Kilpelainen, A.Karolinska Inst, Sweden
(författare)
-
Jansons, J.Riga Stradins Univ, Latvia
(författare)
-
Latyshev, O. E.Gamaleja Res Ctr Epidemiol and Microbiol, Russia; Russian Acad Sci, Russia
(författare)
-
Kuzmenko, Y. V.Russian Acad Sci, Russia
(författare)
-
Hinkula, JormaLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten,Karolinska Inst, Sweden(Swepub:liu)jorhi40
(författare)
-
Abakumov, M. A.Pirogov Russian Natl Res Med Univ, Russia; Natl Univ Sci and Technol MISIS, Russia
(författare)
-
Valuev-Elliston, V. T.Russian Acad Sci, Russia
(författare)
-
Gomelsky, M.Karolinska Institutet,Univ Wyoming, WY 82071 USA,Gamaleja Res Ctr Epidemiol and Microbiol, Russia; Russian Acad Sci, Russia; Riga Stradins Univ, Latvia
(författare)
-
Karpov, V. L.Russian Acad Sci, Russia
(författare)
-
Chiodi, F.Karolinska Institutet,Karolinska Inst, Sweden
(författare)
-
Wahren, B.Karolinska Institutet,Karolinska Inst, Sweden
(författare)
-
Logunov, D. Y.Gamaleja Res Ctr Epidemiol and Microbiol, Russia; Russian Acad Sci, Russia
(författare)
-
Starodubova, E. S.Russian Acad Sci, Russia
(författare)
-
Isaguliants, M. G.Gamaleja Res Ctr Epidemiol and Microbiol, Russia; Russian Acad Sci, Russia; Riga Stradins Univ, Latvia
(författare)
-
Karolinska InstitutetRussian Acad Sci, Russia; Karolinska Inst, Sweden; Gamaleja Res Ctr Epidemiol and Microbiol, Russia
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Scientific Reports: NATURE PUBLISHING GROUP82045-2322
Internetlänk
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Latanova, A. A.
-
Petkov, S.
-
Kilpelainen, A.
-
Jansons, J.
-
Latyshev, O. E.
-
Kuzmenko, Y. V.
-
visa fler...
-
Hinkula, Jorma
-
Abakumov, M. A.
-
Valuev-Elliston, ...
-
Gomelsky, M.
-
Karpov, V. L.
-
Chiodi, F.
-
Wahren, B.
-
Logunov, D. Y.
-
Starodubova, E. ...
-
Isaguliants, M. ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Mikrobiologi ino ...
- Artiklar i publikationen
-
Scientific Repor ...
- Av lärosätet
-
Linköpings universitet
-
Karolinska Institutet