Preparation of functionalized protein materials assisted by mechanochemistry

• Herein, we investigate the suitability of hen egg-white lysozyme (HEWL) as a protein matrix for dispersal of various hydrophobic dyes. Moreover, we investigate the use of a mixer mill for grinding operation as an alternative to hand grinding by mortar and pestle. HEWL and various dyes are mixed by mechanochemistry, and the resulting composite material is dissolved in aqueous acid. The samples are then exposed to conditions promoting self-assembly of HEWL into protein nanofibrils (PNFs). The effect of PNF formation on dye photophysics is investigated by spectroscopic examination by absorption and luminescence spectroscopy, and product morphology is examined by scanning electron microscopy. The self-assembly process results in protein nanofibrils functionalized with luminescent dyes. Such structures may find future applications in various devices for light emission. In addition, we demonstrate that the anticancer drug camptothecin can be incorporated into protein nanofibrils giving materials that can find application as drug delivery agents.

Ämnesord

NATURVETENSKAP  -- Kemi -- Polymerkemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Polymer Chemistry (hsv//eng)

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)