TCF/LEF dependent and independent transcriptional regulation of Wnt/beta-catenin target genes

Nestor, Colm (författare): Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten

Cantù, Claudio (författare): Linköpings universitet,Avdelningen för mikrobiologi, infektion och inflammation,Medicinska fakulteten,Univ Zurich, Switzerland

(creator_code:org_t)

Relaterad länk:: https://onlinelibrar...; visa fler...; https://urn.kb.se/re...; https://doi.org/10.1...; visa färre...

Abstract Ämnesord

Stäng

During canonical Wnt signalling, the activity of nuclear beta-catenin is largely mediated by the TCF/LEF family of transcription factors. To challenge this view, we used the CRISPR/Cas9 genome editing approach to generate HEK 293T cell clones lacking all four TCF/LEF genes. By performing unbiased whole transcriptome sequencing analysis, we found that a subset of beta-catenin transcriptional targets did not require TCF/LEF factors for their regulation. Consistent with this finding, we observed in a genome-wide analysis that beta-catenin occupied specific genomic regions in the absence of TCF/LEF. Finally, we revealed the existence of a transcriptional activity of beta-catenin that specifically appears when TCF/LEF factors are absent, and refer to this as beta-catenin-GHOST response. Collectively, this study uncovers a previously neglected modus operandi of beta-catenin that bypasses the TCF/LEF transcription factors.

Av författaren/redakt...: Doumpas, Nikolao ...; Lampart, Franzis ...; Robinson, Mark D ...; Lentini, Antonio; Nestor, Colm; Cantù, Claudio; visa fler...; Basler, Konrad; visa färre...

Om ämnet

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