Sökning: L773:0271 3683 OR L773:1460 2202 >
Evaluation of the S...
-
Spinozzi, DanieleNetherlands Inst Innovat Ocular Surg, Netherlands
(författare)
Evaluation of the Suitability of Biocompatible Carriers as Artificial Transplants Using Cultured Porcine Corneal Endothelial Cells
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
-
2018-12-07
-
TAYLOR & FRANCIS INC,2019
-
electronicrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:liu-155534
-
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-155534URI
-
https://doi.org/10.1080/02713683.2018.1536215DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Funding Agencies|European Unions Horizon 2020 research and innovation programme [667400]
-
Purpose/Aim: Evaluating the suitability of bioengineered collagen sheets and human anterior lens capsules (HALCs) as carriers for cultivated porcine corneal endothelial cells (pCECs) and in vitro assessment of the cell-carrier sheets as tissue-engineered grafts for Descemet membrane endothelial keratoplasty (DMEK). Materials and Methods: pCECs were isolated, cultured up to P2 and seeded onto LinkCell (TM) bioengineered matrices of 20 mu m (LK20) or 100 mu m (LK100) thickness, and on HALC. During expansion, pCEC viability and morphology were assessed by light microscopy. ZO-1 and Na+/K+-ATPase expression was investigated by immunohistochemistry. Biomechanical properties of pCEC-carrier constructs were evaluated by simulating DMEK surgery in vitro using an artificial anterior chamber (AC) and a human donor cornea without Descemet membrane (DM). Results: During in vitro expansion, cultured pCECs retained their proliferative capacity, as shown by the positive staining for proliferative marker Ki67, and a high cell viability rate (96 +/- 5%). pCECs seeded on all carriers formed a monolayer of hexagonal, tightly packed cells that expressed ZO-1 and Na+/K+-ATPase. During in vitro surgery, pCEC-LK20 and pCEC-LK100 constructs were handled like Descemet stripping endothelial keratoplasty (DSEK) grafts, i.e. folded like a "taco" for insertion because of challenges related to rolling and sticking of the grafts in the injector. pCEC-HALC constructs behaved similar to the DMEK reference model during implantation and unfolding in the artificial AC, showing good adhesion to the bare stroma. Conclusions: In vitro DMEK surgery showed HALC as the most suitable carrier for cultivated pCECs with good intraoperative graft handling. LK20 carrier showed good biocompatibility, but required a DSEK-adapted surgical protocol. Both carriers might be notional candidates for potential future clinical applications.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Miron, AlinaNetherlands Inst Innovat Ocular Surg, Netherlands
(författare)
-
Bruinsma, MariekeNetherlands Inst Innovat Ocular Surg, Netherlands
(författare)
-
Dapena, IsabelNetherlands Inst Innovat Ocular Surg, Netherlands; Melles Cornea Clin Rotterdam, Netherlands
(författare)
-
Lavy, ItayNetherlands Inst Innovat Ocular Surg, Netherlands; Melles Cornea Clin Rotterdam, Netherlands
(författare)
-
Binder, Perry S.Univ Calif Irvine, CA USA
(författare)
-
Rafat, MehrdadLinköpings universitet,Avdelningen för medicinsk teknik,Tekniska fakulteten,LinkoCare Life Sci AB, Linkoping, Sweden(Swepub:liu)mehra64
(författare)
-
Oellerich, SilkeNetherlands Inst Innovat Ocular Surg, Netherlands
(författare)
-
Melles, Gerrit R. J.Netherlands Inst Innovat Ocular Surg, Netherlands; Melles Cornea Clin Rotterdam, Netherlands; Amnitrans EyeBank Rotterdam, Netherlands
(författare)
-
Netherlands Inst Innovat Ocular Surg, NetherlandsNetherlands Inst Innovat Ocular Surg, Netherlands; Melles Cornea Clin Rotterdam, Netherlands
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Current Eye Research: TAYLOR & FRANCIS INC44:3, s. 243-2490271-36831460-2202
Internetlänk
Hitta via bibliotek
Till lärosätets databas