WFRF:(Wang Xuewen)
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Achieving low-power single-wavelength-pair nanoscopy with NIR-II continuous-wave laser for multi-chromatic probes
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- Guo, Xin (författare)
- South China Normal Univ, South China Acad Adv Optoelect, Ctr Opt & Electromagnet Res, Natl Ctr Int Res Green Optoelect,Guangdong Prov K, Guangzhou 510006, Peoples R China.
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- Pu, Rui (författare)
- South China Normal Univ, South China Acad Adv Optoelect, Ctr Opt & Electromagnet Res, Natl Ctr Int Res Green Optoelect,Guangdong Prov K, Guangzhou 510006, Peoples R China.
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- Zhu, Zhimin (författare)
- South China Normal Univ, South China Acad Adv Optoelect, Ctr Opt & Electromagnet Res, Natl Ctr Int Res Green Optoelect,Guangdong Prov K, Guangzhou 510006, Peoples R China.
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- South China Normal Univ, South China Acad Adv Optoelect, Ctr Opt & Electromagnet Res, Natl Ctr Int Res Green Optoelect,Guangdong Prov K, Guangzhou 510006, Peoples R China Kvant- och biofotonik (creator_code:org_t)
- 2022-05-23
- 2022
- Engelska.
- Ingår i: Nature Communications. - : Springer Nature. - 2041-1723. ; 13:1
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- The authors introduce stimulated-emission induced excitation depletion (STExD) nanoscopy using a single pair of low-power, near-infrared, continue-wave lasers. Emission of multichromatic probes is inhibited by cascade amplified depletion in lanthanide upconversion systems induced by manipulating their common sensitizer. Stimulated emission depletion (STED) microscopy is a powerful diffraction-unlimited technique for fluorescence imaging. Despite its rapid evolution, STED fundamentally suffers from high-intensity light illumination, sophisticated probe-defined laser schemes, and limited photon budget of the probes. Here, we demonstrate a versatile strategy, stimulated-emission induced excitation depletion (STExD), to deplete the emission of multi-chromatic probes using a single pair of low-power, near-infrared (NIR), continuous-wave (CW) lasers with fixed wavelengths. With the effect of cascade amplified depletion in lanthanide upconversion systems, we achieve emission inhibition for a wide range of emitters (e.g., Nd3+, Yb3+, Er3+, Ho3+, Pr3+, Eu3+, Tm3+, Gd3+, and Tb3+) by manipulating their common sensitizer, i.e., Nd3+ ions, using a 1064-nm laser. With NaYF4:Nd nanoparticles, we demonstrate an ultrahigh depletion efficiency of 99.3 +/- 0.3% for the 450 nm emission with a low saturation intensity of 23.8 +/- 0.4 kW cm(-2). We further demonstrate nanoscopic imaging with a series of multi-chromatic nanoprobes with a lateral resolution down to 34 nm, two-color STExD imaging, and subcellular imaging of the immunolabelled actin filaments. The strategy expounded here promotes single wavelength-pair nanoscopy for multi-chromatic probes and for multi-color imaging under low-intensity-level NIR-II CW laser depletion.
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- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
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- Guo, Xin
- Pu, Rui
- Zhu, Zhimin
- Qiao, Shuqian
- Liang, Yusen
- Huang, Bingru
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- Liu, Haichun
- Labrador-Páez, L ...
- Kostiv, Uliana
- Zhao, Pu
- Wu, Qiusheng
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- Zhan, Qiuqiang
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