EGOFET Peptide Aptasensor for Label-Free Detection of Inflammatory Cytokines in Complex Fluids

Berto, MarcelloUniv Modena and Reggio Emilia, Italy; Univ Ferrara, Italy (författare)

Funding Agencies|IT MIUR Bilateral Project Italy/Sweden "Poincare"; INFM-CNR Project "EOS - Organic electronics for innovative measuring instruments"; Life Science Department through "FAR"; "Fondazione di Vignola"
Organic electronic transistors are rapidly emerging as ultrahigh sensitive label-free biosensors suited for point-of-care or in-field deployed applications. Most organic biosensors reported to date are based on immunorecognition between the relevant biomarkers and the immobilized antibodies, whose use is hindered by large dimensions, poor control of sequence, and relative instability. Here, an electrolyte-gated organic field effect transistor (EGOFET) biosensor where the recognition units are surface immobilized peptide aptamers (Affimer proteins) instead of antibodies is reported. Peptide aptasensor for the detection of the pro-inflammatory cytokine tumor necrosis factor alpha (TNF alpha) with a 1 x 10(-12) M limit of detection is demonstrated. Ultralow sensitivity is met even in complex solutions such as cell culture media containing 10% serum, demonstrating the remarkable ligand specificity of the device. The device performances, together with the simple one-step immobilization strategy of the recognition moieties and the low operational voltages, all prompt EGOFET peptide aptasensors as candidates for early diagnostics and monitoring at the point-of-care.

NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
Affimer protein; binding constant; organic bioelectronics; peptide aptamers; TNF alpha

Diacci, ChiaraUniv Modena and Reggio Emilia, Italy (författare)
DAgata, RobertaUniv Catania, Italy (författare)
Pinti, MarcelloUniv Modena and Reggio Emilia, Italy (författare)
Bianchini, ElenaUniv Modena and Reggio Emilia, Italy (författare)
Di Lauro, MicheleUniv Modena and Reggio Emilia, Italy (författare)
Casalini, StefanoUniv Modena and Reggio Emilia, Italy; Inst Ciencia Mat Barcelona ICMAB CSIC, Spain (författare)
Cossarizza, AndreaUniv Modena and Reggio Emilia, Italy (författare)
Berggren, MagnusLinköpings universitet,Laboratoriet för organisk elektronik,Tekniska fakulteten(Swepub:liu)magbe98 (författare)
Simon, DanielLinköpings universitet,Laboratoriet för organisk elektronik,Tekniska fakulteten(Swepub:liu)dansi08 (författare)
Spoto, GiuseppeUniv Catania, Italy; Univ Catania, Italy (författare)
Biscarini, FabioUniv Modena and Reggio Emilia, Italy (författare)
Bortolotti, Carlo A.Univ Modena and Reggio Emilia, Italy (författare)
Univ Modena and Reggio Emilia, Italy; Univ Ferrara, ItalyUniv Modena and Reggio Emilia, Italy (creator_code:org_t)

Av författaren/redakt...: Berto, Marcello; Diacci, Chiara; DAgata, Roberta; Pinti, Marcello; Bianchini, Elena; Di Lauro, Michel ...; visa fler...; Casalini, Stefan ...; Cossarizza, Andr ...; Berggren, Magnus; Simon, Daniel; Spoto, Giuseppe; Biscarini, Fabio; Bortolotti, Carl ...; visa färre...

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