Sökning: WFRF:(King Zachary) >
Cystic fibrosis tra...
Cystic fibrosis transmembrane conductance regulator modulates enteric cholinergic activities and is abnormally expressed in the enteric ganglia of patients with slow transit constipation
-
- Yeh, Ka Ming (författare)
- Univ New South Wales, Australia
-
- Johansson, Olle (författare)
- Linköpings universitet,Medicinska fakulteten
-
- Le, Huy (författare)
- Univ New South Wales, Australia
-
visa fler...
-
- Rao, Karan (författare)
- Univ New South Wales, Australia
-
- Markus, Irit (författare)
- Univ New South Wales, Australia
-
- Perera, Dayashan Shevy (författare)
- Sydney Colorectal Associates, Australia
-
- Lubowski, David Zachary (författare)
- Sydney Colorectal Associates, Australia
-
- King, Denis Warwick (författare)
- Sydney Colorectal Associates, Australia
-
- Zhang, Li (författare)
- Univ New South Wales, Australia
-
- Chen, Hongzhuan (författare)
- Shanghai Jiao Tong Univ, Peoples R China
-
- Liu, Lu (författare)
- Univ New South Wales, Australia
-
visa färre...
-
(creator_code:org_t)
- 2019-08-07
- 2019
- Engelska.
-
Ingår i: Journal of gastroenterology. - : SPRINGER JAPAN KK. - 0944-1174 .- 1435-5922. ; 54:11, s. 994-1006
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background Cystic fibrosis transmembrane conductance regulator (CFTR) was recently found in the enteric nervous system, where its role is unclear. We aimed to identify which enteric neuronal structures express CFTR, whether CFTR modulates enteric neurotransmission and if altered CFTR expression is associated with slow transit constipation (STC). Methods Immunofluorescence double labeling was performed to localize CFTR with various neuronal and glial cell markers in the human colon. The immunoreactivity (IR) of CFTR and choline acetyltransferase (ChAT) on myenteric plexus of control and STC colon was quantitatively analyzed. In control colonic muscle strips, electrical field stimulation (EFS) evoked contractile responses and the release of acetylcholine (ACh) was measured in the presence of the CFTR channel inhibitor, CFTR(inh)-172. Results CFTR-IR was densely localized to myenteric ganglia, where it was co-localized with neuronal markers HuC/D and beta-tubulin, and glial marker S-100 but little with glial fibrillary acidic protein. Vesicular ACh transport was almost exclusively co-localized with CFTR, but neurons expressing nitric oxide synthase were CFTR negative. Significant reductions of CFTR-IR (P amp;lt; 0.01) and ChAT-IR (P amp;lt; 0.05) were observed on myenteric ganglia of STC compared to control. Pre-treatment of colonic muscle strips with CFTR(inh)-172 (10 mu M) significantly inhibited EFS-evoked contractile responses (P amp;lt; 0.01) and ACh release (P amp;lt; 0.05). Conclusions Co-localization of CFTR-IR with cholinergic markers, inhibition of EFS-induced colonic muscle contractility and ACh release by CFTR(inh)-172 suggest that CFTR modulates enteric cholinergic neurotransmission. The downregulation of CFTR and ChAT in myenteric ganglia of STC correlated with the impaired contractile responses to EFS.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
Nyckelord
- CFTR; Motility disorder; Slow transit constipation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Yeh, Ka Ming
-
Johansson, Olle
-
Le, Huy
-
Rao, Karan
-
Markus, Irit
-
Perera, Dayashan ...
-
visa fler...
-
Lubowski, David ...
-
King, Denis Warw ...
-
Zhang, Li
-
Chen, Hongzhuan
-
Liu, Lu
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Gastroenterologi
- Artiklar i publikationen
-
Journal of gastr ...
- Av lärosätet
-
Linköpings universitet