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Seminal Plasma Modi...
Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs
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- Martinez-Serrano, Cristina (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain
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- Cambra, Josep M. (författare)
- Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain
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- Parrilla, Inmaculada (författare)
- Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain
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- Roca, Jordi (författare)
- Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain
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- Ferreira-Dias, Graca (författare)
- Univ Lisbon, Portugal
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- Pallares, Francisco J. (författare)
- Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain
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- Lucas, Xiomara (författare)
- Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain
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- Vazquez, Juan M. (författare)
- Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain
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- Martinez, Emilio A. (författare)
- Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain
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- Gil, Maria A. (författare)
- Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain
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- Rodriguez-Martinez, Heriberto (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten
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- Cuello, Cristina (författare)
- Univ Murcia, Spain; Inst Biomed Res Murcia IMIB Arrixaca, Spain
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- Alvarez-Rodriguez, Manuel (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten
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(creator_code:org_t)
- 2019-12-18
- 2019
- Engelska.
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Ingår i: Frontiers in Veterinary Science. - : Frontiers Media S.A.. - 2297-1769. ; 6
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://doi.org/10.3...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Background: Boar seminal plasma is rich in cytokines, which could influence the capability of spermatozoa to tolerate preservation.Objectives: To evaluate the involvement of boar seminal plasma cytokines in the changes experienced by boar spermatozoa during their storage, either in liquid or frozen state.Materials and Methods: In two separated experiments, semen samples from healthy and fertile boars were split in two aliquots, one centrifuged twice (1,500 ×g for 10 min) to harvest seminal plasma, whereas the other was either commercially extended (3 × 107 sperm/mL) and liquid-stored at 17°C during 144 h (n = 28, Experiment 1) or frozen-thawed using a standard 0.5 mL protocol (n = 27, Experiment 2). Sixteen cytokines were quantified using Luminex xMAP®. Sperm attributes (CASA-evaluated total and progressive motility; flow cytometry-evaluated sperm viability, production of intracellular H2O2 and O2•-" role="presentation" style="box-sizing: border-box; display: inline; line-height: normal; word-spacing: normal; overflow-wrap: normal; white-space: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; border: 0px; padding: 0px; margin: 0px; position: relative; outline: 0px !important;">O∙−2O2•- and levels of lipid peroxidation in viable spermatozoa) were evaluated either at 0, 72, or 144 h of liquid storage (Experiment 1) or before freezing and at 30- and 150-min post-thawing (Experiment 2).Results: Multiple linear regression models, with Bayesian approach for variable selection, revealed that the anti-inflammatory TGF-β2, TGF-β3, IL-1Ra, and IL-4 and the pro-inflammatory IL-8 and IL-18, predicted changes in sperm motility for liquid-stored semen while the anti-inflammatory IFN-γ was included in the models predicting changes in all sperm attributes for cryopreserved semen.Conclusion: Specific boar seminal plasma cytokines would contribute to modulate the structural and metabolic changes shown by spermatozoa during preservation, either in liquid or frozen state.
Ämnesord
- NATURVETENSKAP -- Biologi -- Utvecklingsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Developmental Biology (hsv//eng)
Nyckelord
- seminal plasma; endometrium; transcriptome; embryo; preimplantation; pig
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Martinez-Serrano ...
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Cambra, Josep M.
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Parrilla, Inmacu ...
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Roca, Jordi
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Ferreira-Dias, G ...
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Pallares, Franci ...
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visa fler...
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Lucas, Xiomara
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Vazquez, Juan M.
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Martinez, Emilio ...
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Gil, Maria A.
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Rodriguez-Martin ...
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Cuello, Cristina
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Alvarez-Rodrigue ...
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visa färre...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Utvecklingsbiolo ...
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Frontiers in Vet ...
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Linköpings universitet