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Sökning: WFRF:(Slater Sarah) > (2020) > High Cysteinyl Leuk...

  • Slater, KayleighUniv Coll Dublin, Ireland; Genom Med Ireland Ltd, Ireland (författare)

High Cysteinyl Leukotriene Receptor 1 Expression Correlates with Poor Survival of Uveal Melanoma Patients and Cognate Antagonist Drugs Modulate the Growth, Cancer Secretome, and Metabolism of Uveal Melanoma Cells

  • Artikel/kapitelEngelska2020

Förlag, utgivningsår, omfång ...

  • 2020-10-13
  • MDPI,2020
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:liu-171483
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-171483URI
  • https://doi.org/10.3390/cancers12102950DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Funding Agencies|Irish Research CouncilIrish Research Council for Science, Engineering and Technology [EBP/2017/473]; British Pharmacological Society; Breakthrough Cancer Research; European UnionEuropean Union (EU) [734907]; Science Foundation IrelandScience Foundation Ireland [15/IA/3104, 18/SPP/3522]
  • Simple Summary This research investigates the disease relevance and therapeutic potential of cysteinyl leukotriene receptors in uveal melanoma (UM), a rare eye cancer that often spreads to the liver. Unfortunately, there are no therapies available to stop the spread of UM and patients are often faced with an extremely poor prognosis. We assess whether the cysteinyl leukotriene receptors (CysLT(1) and CysLT(2)) are relevant to the progression of UM. Using UM patient samples, we identified that increased levels of CysLT(1) in tumours is associated with reduced patient survival. Using UM cell lines and zebrafish models, we found that drugs targeting CysLT(1), but not CysLT(2), can alter hallmarks of cancer including cell growth, proliferation, and metabolism. This study is the first to examine the relationship of the CysLT receptors with clinical features of UM. Our data strengthen the importance of CysLT signalling in UM and suggest that antagonism of CysLT(1) may be of therapeutic interest in the disease. Metastatic uveal melanoma (UM) is a rare, but often lethal, form of ocular cancer arising from melanocytes within the uveal tract. UM has a high propensity to spread hematogenously to the liver, with up to 50% of patients developing liver metastases. Unfortunately, once liver metastasis occurs, patient prognosis is extremely poor with as few as 8% of patients surviving beyond two years. There are no standard-of-care therapies available for the treatment of metastatic UM, hence it is a clinical area of urgent unmet need. Here, the clinical relevance and therapeutic potential of cysteinyl leukotriene receptors (CysLT(1) and CysLT(2)) in UM was evaluated. High expression of CYSLTR1 or CYSLTR2 transcripts is significantly associated with poor disease-free survival and poor overall survival in UM patients. Digital pathology analysis identified that high expression of CysLT(1) in primary UM is associated with reduced disease-specific survival (p = 0.012; HR 2.76; 95% CI 1.21-6.3) and overall survival (p = 0.011; HR 1.46; 95% CI 0.67-3.17). High CysLT(1) expression shows a statistically significant (p = 0.041) correlation with ciliary body involvement, a poor prognostic indicator in UM. Small molecule drugs targeting CysLT(1) were vastly superior at exerting anti-cancer phenotypes in UM cell lines and zebrafish xenografts than drugs targeting CysLT(2). Quininib, a selective CysLT(1) antagonist(,) significantly inhibits survival (p < 0.0001), long-term proliferation (p < 0.0001), and oxidative phosphorylation (p < 0.001), but not glycolysis, in primary and metastatic UM cell lines. Quininib exerts opposing effects on the secretion of inflammatory markers in primary versus metastatic UM cell lines. Quininib significantly downregulated IL-2 and IL-6 in Mel285 cells (p < 0.05) but significantly upregulated IL-10, IL-1 beta, IL-2 (p < 0.0001), IL-13, IL-8 (p < 0.001), IL-12p70 and IL-6 (p < 0.05) in OMM2.5 cells. Finally, quininib significantly inhibits tumour growth in orthotopic zebrafish xenograft models of UM. These preclinical data suggest that antagonism of CysLT(1), but not CysLT(2), may be of therapeutic interest in the treatment of UM.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Heeran, Aisling B.Trinity Coll Dublin, Ireland (författare)
  • Garcia-Mulero, SandraBellvitge Biomed Res Inst IDIBELL, Spain; CIBERESP, Spain; Univ Barcelona, Spain (författare)
  • Kalirai, HelenUniv Liverpool, England (författare)
  • Sanz-Pamplona, RebecaBellvitge Biomed Res Inst IDIBELL, Spain; CIBERESP, Spain (författare)
  • Rahman, ArmanUniv Coll Dublin, Ireland (författare)
  • Al-Attar, NebrasUniv Coll Dublin, Ireland (författare)
  • Helmi, MaysLinköpings universitet,Avdelningen för diagnostik och specialistmedicin,Medicinska fakulteten(Swepub:liu)n/a (författare)
  • OConnell, FionaTrinity Coll Dublin, Ireland (författare)
  • Bosch, RosaXenopat SL, Spain (författare)
  • Portela, AnnaXenopat SL, Spain (författare)
  • Villanueva, AlbertoXenopat SL, Spain (författare)
  • Gallagher, William M.Univ Coll Dublin, Ireland (författare)
  • Jensen, LasseLinköpings universitet,Avdelningen för diagnostik och specialistmedicin,Medicinska fakulteten,Region Östergötland, Klinisk farmakologi(Swepub:liu)larje86 (författare)
  • Piulats, Josep M.IDIBELL OncoBell, Spain; CIBERONC, Spain (författare)
  • Coupland, Sarah E.Univ Liverpool, England; Liverpool Univ Hosp NHS Fdn Trust, England (författare)
  • OSullivan, JacinthaTrinity Coll Dublin, Ireland (författare)
  • Kennedy, Breandan N.Univ Coll Dublin, Ireland (författare)
  • Univ Coll Dublin, Ireland; Genom Med Ireland Ltd, IrelandTrinity Coll Dublin, Ireland (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Cancers: MDPI12:102072-6694

Internetlänk

Hitta via bibliotek

  • Cancers (Sök värdpublikationen i LIBRIS)

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