Simultaneous Measurement of 11 Antibiotics for use in the Intensive Care Unit by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

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Woksepp, HannaDepartment of Clinical Microbiology, Kalmar County Hospital, Kalmar, Sweden; Department of Medicine and Optometry, Linnaeus University, Kalmar, Sweden (author)

Simultaneous Measurement of 11 Antibiotics for use in the Intensive Care Unit by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

Article/chapterEnglish2022

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Philadelphia, PA, United States :Lippincott Williams & Wilkins,2022
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LIBRIS-ID:oai:DiVA.org:liu-183609
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-183609URI
https://doi.org/10.1097/ftd.0000000000000911DOI

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Language:English
Summary in:English

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Subject category:art swepub-publicationtype

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Funding: Research Council of South-East Sweden (FORSS); Swedish Research CouncilSwedish Research CouncilEuropean Commission; Marianne and Marcus Wallenberg Foundation [DNR 2014/236-31]
BACKGROUND: Recent studies indicate that a high proportion of patients in the intensive care unit (ICU) fail to attain adequate antibiotic levels. Thus, there is a need to monitor the antibiotic concentration to ensure effective treatment. Herein, the authors aimed to develop an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous quantification of antimicrobials to assess individualized therapeutic drug monitoring (TDM).METHODS: A UHPLC-MS/MS method with 11 antibiotics (ciprofloxacin, moxifloxacin, benzylpenicillin, levofloxacin, linezolid, rifampicin, meropenem, cloxacillin, cefotaxime, clindamycin, and piperacillin) was developed. Chromatographic separation was performed using a Kinetex biphenyl reversed-phase column, with gradient elution using 0.1% formic acid (FA) and methanol with 0.1% FA. Sample preparation was performed using methanol protein precipitation. The total run time was 5 min.RESULTS: For all analytes, the inter-assay inaccuracies for calibrators were ≤5%. The inter-day inaccuracies for the quality controls (QCs) were ≤5% for all analytes. The inter-assay precision for calibration standards ranged between 1.42% and 6.11%. The inter-assay imprecision for QCs of all antibiotics and concentrations ranged between 3.60% and 16.1%. Inter-assay inaccuracy and imprecision for the QCs and calibration standards were ≤15% for all drugs, except benzylpenicillin.CONCLUSION: A rapid UHPLC-MS/MS method was developed for the simultaneous quantification of 11 different antibiotics. Minimal sample preparation was required to ensure a rapid turnaround time. The method was applied to clinical samples collected from four ICUs.

Subject headings and genre

NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
UHPLC-MS/MS; Antibiotics; Therapeutic drug monitoring; Critically ill patients; Clinical application

Added entries (persons, corporate bodies, meetings, titles ...)

Karlsson, Louise,1981-Linköpings universitet,Avdelningen för klinisk kemi och farmakologi,Medicinska fakulteten,Region Östergötland, Klinisk farmakologi(Swepub:liu)louka51 (author)
Ärlemalm, AndreasLinköpings universitet,Avdelningen för klinisk kemi och farmakologi,Medicinska fakulteten,Region Östergötland, Klinisk farmakologi(Swepub:liu)n/a (author)
Hällgren, Anita,1963-Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Region Östergötland, Infektionskliniken i Östergötland(Swepub:liu)aniha32 (author)
Schön, Thomas,1973-Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Region Östergötland, Infektionskliniken i Östergötland,Department of Infectious Diseases, Kalmar County Hospital, Kalmar, Sweden(Swepub:liu)thosc49 (author)
Carlsson, Björn,1958-Linköpings universitet,Avdelningen för klinisk kemi och farmakologi,Medicinska fakulteten,Region Östergötland, Klinisk farmakologi(Swepub:liu)bjoca98 (author)
Department of Clinical Microbiology, Kalmar County Hospital, Kalmar, Sweden; Department of Medicine and Optometry, Linnaeus University, Kalmar, SwedenAvdelningen för klinisk kemi och farmakologi (creator_code:org_t)

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In:Therapeutic Drug MonitoringPhiladelphia, PA, United States : Lippincott Williams & Wilkins44:2, s. 308-3180163-43561536-3694

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