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Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids

Bojmar, Linda, 1983- (author)
Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
Kim, Han Sang (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Yonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea
Tobias, Gabriel C (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil
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Pelissier Vatter, Fanny A (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
Lucotti, Serena (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
Gyan, Kofi Ennu (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Tri-Institutional PhD Program in Computational Biology and Medicine, New York, NY, USA
Kenific, Candia M. (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
Wan, Zurong (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Department of Pediatrics, Weill Cornell Medicine, New York, NY, USA
Kim, Kyung-A (author)
Yonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea
Kim, DooA (author)
Yonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea
Hernandez, Jonathan (author)
Hepatopancreatobiliary Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA; Surgical Oncology Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
Pascual, Virginia (author)
Department of Pediatrics, Weill Cornell Medicine, New York, NY, USA; Drukier Institute for Childrens Health, Weill Cornell Medicine, New York, NY, USA
Heaton, Todd E (author)
Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA; Pediatric Surgical Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA
La Quaglia, Michael P (author)
Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA; Pediatric Surgical Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA
Kelsen, David (author)
Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA
Trippett, Tanya M (author)
Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA
Jones, David R (author)
Thoracic Surgery Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA
Jarnagin, William R. (author)
Hepatopancreatobiliary Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA
Matei, Irina R. (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
Zhang, Haiying (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
Hoshino, Ayuko (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan
Lyden, David (author)
Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
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 (creator_code:org_t)
Cell Press, 2021
2021
English.
In: STAR protocols. - : Cell Press. - 2666-1667. ; 2:1
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling. For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020).

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)

Keyword

Cell Membrane; Cell culture; Cell isolation; Exomeres; Exosomes; Extracellular vesicles and particles; Isolation

Publication and Content Type

ref (subject category)
art (subject category)

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