Sökning: id:"swepub:oai:DiVA.org:liu-186167" >
Amyloidogenesis of ...
-
Nyström, SofieLinköpings universitet,Kemi,Tekniska fakulteten
(författare)
Amyloidogenesis of SARS-CoV-2 Spike Protein br
- Artikel/kapitelEngelska2022
Förlag, utgivningsår, omfång ...
-
2022-05-17
-
AMER CHEMICAL SOC,2022
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:liu-186167
-
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-186167URI
-
https://doi.org/10.1021/jacs.2c03925DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Funding Agencies|Swedish Research Council [2019-04405]
-
SARS-CoV-2 infection is associated with a surprising number of morbidities. Uncanny similarities with amyloid-disease associated blood coagulation andfibrinolytic disturbances together with neurologic and cardiac problems led us to investigatethe amyloidogenicity of the SARS-CoV-2 spike protein (S-protein). Amyloidfibril assays of peptide library mixtures and theoreticalpredictions identified seven amyloidogenic sequences within the S-protein. All seven peptides in isolation formed aggregates duringincubation at 37 degrees C. Three 20-amino acid long synthetic spike peptides (sequence 192-211, 601-620, 1166-1185) fulfilled threeamyloidfibril criteria: nucleation dependent polymerization kinetics by ThT, Congo red positivity, and ultrastructuralfibrillarmorphology. Full-length folded S-protein did not form amyloidfibrils, but amyloid-likefibrils with evident branching were formedduring 24 h of S-protein coincubation with the protease neutrophil elastase (NE)in vitro.NEefficiently cleaved S-protein, renderingexposure of amyloidogenic segments and accumulation of the amyloidogenic peptide 194-203, part of the most amyloidogenicsynthetic spike peptide. NE is overexpressed at inflamed sites of viral infection. Our data propose a molecular mechanism forpotential amyloidogenesis of SARS-CoV-2 S-protein in humans facilitated by endoproteolysis. The prospective of S-proteinamyloidogenesis in COVID-19 disease associated pathogenesis can be important in understanding the disease and long COVID-19.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Hammarström, PerLinköpings universitet,Kemi,Tekniska fakulteten(Swepub:liu)perha81
(författare)
-
Linköpings universitetKemi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Journal of the American Chemical Society: AMER CHEMICAL SOC144:20, s. 8945-89500002-78631520-5126
Internetlänk
Hitta via bibliotek
Till lärosätets databas