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Epigenetic rewiring...
Epigenetic rewiring of pathways related to odour perception in immune cells exposed to SARS-CoV-2 in vivo and in vitro
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- Huoman, Johanna (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Univ Hosp Bonn, Germany
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- Sayyab, Shumaila (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
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- Apostolou, Eirini (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
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- Karlsson, Lovisa (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
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- Porcile, Lucas (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
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- Rizwan, Muhammad (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
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- Sharma, Sumit (författare)
- Linköpings universitet,Avdelningen för molekylär medicin och virologi,Medicinska fakulteten
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- Das, Jyotirmoy (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
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- Rosén, Anders (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
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- Lerm, Maria (författare)
- Linköpings universitet,Avdelningen för inflammation och infektion,Medicinska fakulteten
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(creator_code:org_t)
- 2022-06-26
- 2022
- Engelska.
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Ingår i: Epigenetics. - New York, NY, United States : Taylor & Francis. - 1559-2294 .- 1559-2308. ; 17:13, s. 1875-1891
- Relaterad länk:
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https://doi.org/10.1...
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A majority of SARS-CoV-2 recoverees develop only mild-to-moderate symptoms, while some remain completely asymptomatic. Although viruses, including SARS-CoV-2, may evade host immune responses by epigenetic mechanisms including DNA methylation, little is known about whether these modifications are important in defence against and healthy recovery from COVID-19 in the host. To this end, epigenome-wide DNA methylation patterns from COVID-19 convalescents were compared to uninfected controls from before and after the pandemic. Peripheral blood mononuclear cell (PBMC) DNA was extracted from uninfected controls, COVID-19 convalescents, and symptom-free individuals with SARS-CoV-2-specific T cell-responses, as well as from PBMCs stimulated in vitro with SARS-CoV-2. Subsequently, the Illumina MethylationEPIC 850K array was performed, and statistical/bioinformatic analyses comprised differential DNA methylation, pathway over-representation, and module identification analyses. Differential DNA methylation patterns distinguished COVID-19 convalescents from uninfected controls, with similar results in an experimental SARS-CoV-2 infection model. A SARS-CoV-2-induced module was identified in vivo, comprising 66 genes of which six (TP53, INS, HSPA4, SP1, ESR1, and FAS) were present in corresponding in vitro analyses. Over-representation analyses revealed involvement in Wnt, muscarinic acetylcholine receptor signalling, and gonadotropin-releasing hormone receptor pathways. Furthermore, numerous differentially methylated and network genes from both settings interacted with the SARS-CoV-2 interactome. Altered DNA methylation patterns of COVID-19 convalescents suggest recovery from mild-to-moderate SARS-CoV-2 infection leaves longstanding epigenetic traces. Both in vitro and in vivo exposure caused epigenetic modulation of pathways thataffect odour perception. Future studies should determine whether this reflects host-induced protective antiviral defense or targeted viral hijacking to evade host defence.
Ämnesord
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Medicinsk biovetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Medical Bioscience (hsv//eng)
Nyckelord
- SARS-CoV-2; mild-to-moderate; PBMC; DNA methylation; in vitro stimulation; module identification; network analysis; interactome
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Huoman, Johanna
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Sayyab, Shumaila
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Apostolou, Eirin ...
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Karlsson, Lovisa
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Porcile, Lucas
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Rizwan, Muhammad
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visa fler...
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Sharma, Sumit
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Das, Jyotirmoy
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Rosén, Anders
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Lerm, Maria
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visa färre...
- Om ämnet
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- LANTBRUKSVETENSKAPER
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LANTBRUKSVETENSK ...
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och Veterinärmedicin
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och Medicinsk biovet ...
- Artiklar i publikationen
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Epigenetics
- Av lärosätet
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Linköpings universitet