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Sperm freezability ...
Sperm freezability is neither associated with the expression of aquaporin 3 nor sperm head dimensions in dromedary camel (Camelus dromedarius)
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- OBrien, Emma (författare)
- INIA CSIC, Spain
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- Malo, Clara (författare)
- Camel Reprod Ctr, U Arab Emirates
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- Castano, Cristina (författare)
- INIA CSIC, Spain
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- Garcia-Casado, Pedro (författare)
- Zoitechlab Arquimea Grp, Spain
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- Toledano-Diaz, Adolfo (författare)
- INIA CSIC, Spain
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- Martinez-Madrid, Belen (författare)
- Univ Complutense Madrid, Spain
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- Rodriguez-Martinez, Heriberto (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten
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- Alvarez-Rodriguez, Manuel (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,INIA CSIC, Spain
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- Santiago-Moreno, Julian (författare)
- INIA CSIC, Spain
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(creator_code:org_t)
- ELSEVIER SCIENCE INC, 2022
- 2022
- Engelska.
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Ingår i: Theriogenology. - : ELSEVIER SCIENCE INC. - 0093-691X .- 1879-3231. ; 189, s. 230-236
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The expression of aquaglyceroporin 3 (AQP-3) has been demonstrated in the spermatozoa of several mammalian species and its role has been associated with cryotolerance. Post-thaw sperm quality from individual dromedary males with different response to freezing-thawing process was evaluated through sperm head morphometry. In order to understand the cellular mechanisms affected by cryoinjury we have explored the presence and distribution of sperm AQP-3 using western blotting and immunocyto-chemistry. WB showed different intensity of the specific signal bands at 28 kDa. Immunofluorescence assessments allowed us to identify five different and clear AQP-3 distribution patterns of labelling in the sperm plasma membrane; acrosome, post-acrosome, mid-piece, and principal and final tail. Although expression of AQP-3 varied among male ejaculates, the individual sperm response to freeze-thawing was not associated with AQP-3 expression. Thus, AQP3 expressions do not seem like a reliable predictor of sperm response to freeze-thawing process in this species. This work is the first to describe the morphometric characteristics of the heads of dromedary spermatozoa. No correlation was found be-tween sperm head dimensions and sperm quality variables after freeze-thawing suggesting that dromedary camel sperm head morphometry is also not a reliable predictor of cryosurvival.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
Nyckelord
- Dromedary; Cryopreservation; Semen; Aquaglyceroporin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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