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Proteomics reveals ...
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Araújo, Mário JCIIMAR Interdisciplinary Ctr Marine & Environm Re, Portugal
(författare)
Proteomics reveals multiple effects of titanium dioxide and silver nanoparticles in the metabolism of turbot, Scophthalmus maximus
- Artikel/kapitelEngelska2022
Förlag, utgivningsår, omfång ...
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Pergamon-Elsevier Science Ltd,2022
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-189207
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-189207URI
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https://doi.org/10.1016/j.chemosphere.2022.136110DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Funding: European Regional Development Fund (ERDF) through Interreg Atlantic Area Program, project NANO -CULTURE [EAPA 590/2018]; Portuguese Foundation for Science and Technology (Fundacao para a Ciencia e Tecnologia; FCT) [UIDB/04423/2020, UIDP/04423/2020]
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Titanium dioxide (TiO2) and silver (Ag) NPs are among the most used engineered inorganic nanoparticles (NPs); however, their potential effects to marine demersal fish species, are not fully understood. Therefore, this study aimed to assess the proteomic alterations induced by sub-lethal concentrations citrate-coated 25 nm ("P25") TiO2 or polyvinylpyrrolidone (PVP) coated 15 nm Ag NPs to turbot, Scophthalmus maximus. Juvenile fish were exposed to the NPs through daily feeding for 14 days. The tested concentrations were 0, 0.75 or 1.5 mg of each NPs per kg of fish per day. The determination of NPs, Titanium and Ag levels (sp-ICP-MS/ICP-MS) and histological alterations (Transmission Electron Microscopy) supported proteomic analysis performed in the liver and kidney. Proteomic sample preparation procedure (SP3) was followed by LC-MS/MS. Label-free MS quantification methods were employed to assess differences in protein expression. Functional analysis was performed using STRING web-tool. KEGG Gene Ontology suggested terms were discussed and potential biomarkers of exposure were proposed. Overall, data shows that liver accumulated more elements than kidney, presented more histological alterations (lipid droplets counts and size) and proteomic alterations. The Differentially Expressed Proteins (DEPs) were higher in Ag NPs trial. The functional analysis revealed that both NPs caused enrichment of proteins related to generic processes (metabolic pathways). Ag NPs also affected protein synthesis and nucleic acid transcription, among other processes. Proteins related to thyroid hormone transport (Serpina7) and calcium ion binding (FAT2) were suggested as biomarkers of TiO2 NPs in liver. For Ag NPs, in kidney (and at a lower degree in liver) proteins related with metabolic activity, metabolism of exogenous substances and oxidative stress (e.g.: NADH dehydrogenase and Cytochrome P450) were suggested as potential biomarkers. Data suggests adverse effects in turbot after medium/long-term exposures and the need for additional studies to validate specific biological applications of these NPs.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Sousa, Maria LCIIMAR Interdisciplinary Ctr Marine & Environm Re, Portugal
(författare)
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Fonseca, ElzaCIIMAR Interdisciplinary Ctr Marine & Environm Re, Portugal
(författare)
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Felpeto, Aldo BarreiroCIIMAR Interdisciplinary Ctr Marine & Environm Re, Portugal
(författare)
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Martins, José CarlosCIIMAR Interdisciplinary Ctr Marine & Environm Re, Portugal
(författare)
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Vázquez, MaríaCETGA Cluster Acuicultura Galicia, Spain
(författare)
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Mallo, NataliaCETGA Cluster Acuicultura Galicia, Spain
(författare)
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Rodriguez-Lorenzo, LauraINL Int Iberian Nanotechnol Lab, Portugal
(författare)
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Quarato, MonicaINL Int Iberian Nanotechnol Lab, Portugal
(författare)
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Pinheiro, IvoneINL Int Iberian Nanotechnol Lab, Portugal
(författare)
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Turkina, Maria V,1973-Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten(Swepub:liu)martu07
(författare)
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López-Mayán, Juan JoséUniv Santiago de Compostela, Spain
(författare)
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Peña-Vázquez, ElenaUniv Santiago de Compostela, Spain
(författare)
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Barciela-Alonso, María CarmenUniv Santiago de Compostela, Spain
(författare)
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Spuch-Calvar, MiguelUniv Vigo, Spain
(författare)
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Oliveira, MiguelUniv Aveiro, Portugal
(författare)
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Bermejo-Barrera, PilarUniv Santiago de Compostela, Spain
(författare)
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Cabaleiro, SantiagoCETGA Cluster Acuicultura Galicia, Spain
(författare)
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Espiña, BegoñaINL Int Iberian Nanotechnol Lab, Portugal
(författare)
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Vasconcelos, VitorCIIMAR Interdisciplinary Ctr Marine & Environm Re, Portugal; Univ Porto, Portugal
(författare)
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Campos, AlexandreCIIMAR Interdisciplinary Ctr Marine & Environm Re, Portugal
(författare)
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CIIMAR Interdisciplinary Ctr Marine & Environm Re, PortugalCETGA Cluster Acuicultura Galicia, Spain
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Chemosphere: Pergamon-Elsevier Science Ltd3080045-65351879-1298
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Till lärosätets databas
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Araújo, Mário J
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Sousa, Maria L
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Fonseca, Elza
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Felpeto, Aldo Ba ...
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Martins, José Ca ...
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Vázquez, María
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visa fler...
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Mallo, Natalia
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Rodriguez-Lorenz ...
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Quarato, Monica
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Pinheiro, Ivone
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Turkina, Maria V ...
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López-Mayán, Jua ...
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Peña-Vázquez, El ...
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Barciela-Alonso, ...
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Spuch-Calvar, Mi ...
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Oliveira, Miguel
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Bermejo-Barrera, ...
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Cabaleiro, Santi ...
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Espiña, Begoña
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Vasconcelos, Vit ...
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Campos, Alexandr ...
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