Long-distance turgor pressure changes induce local activation of plant glutamate receptor-like channels

Funding Agencies|Piattaforma Piante
In Arabidopsis thaliana, local wounding and herbivore feeding provoke leaf-to-leaf propagating Ca2+ waves that are dependent on the activity of members of the glutamate receptor-like channels (GLRs). In systemic tissues, GLRs are needed to sustain the synthesis of jasmonic acid (JA) with the subsequent activation of JA dependent signaling response required for the plant acclimation to the perceived stress. Even though the role of GLRs is well established, the mechanism through which they are activated remains unclear. Here, we report that in vivo, the amino-acid-dependent activation of the AtGLR3.3 channel and systemic responses require a functional ligand-binding domain. By combining imaging and genetics, we show that leaf mechanical injury, such as wounds and burns, as well as hypo-osmotic stress in root cells, induces the systemic apoplastic increase of L-glutamate (L-Glu), which is largely independent of AtGLR3.3 that is instead required for systemic cytosolic Ca2+ elevation. Moreover, by using a bioelectronic approach, we show that the local release of minute concentrations of L-Glu in the leaf lamina fails to induce any long-distance Ca2+ waves.

Buratti, StefanoUniv Milan, Italy (författare)
Parmagnani, Ambra SeleneUniv Milan, Italy (författare)
Abdel Aziz, IlariaLinköpings universitet,Laboratoriet för organisk elektronik,Tekniska fakulteten(Swepub:liu)ilaab80 (författare)
Bernacka Wojcik, IwonaLinköpings universitet,Laboratoriet för organisk elektronik,Tekniska fakulteten(Swepub:liu)iwobe83 (författare)
Resentini, FrancescaUniv Milan, Italy (författare)
Simura, JanSwedish Univ Agr Sci, Sweden (författare)
Doccula, Fabrizio GandolfoUniv Milan, Italy (författare)
Alfieri, AndreaUniv Milan, Italy; Univ Pavia, Italy (författare)
Luoni, LauraUniv Milan, Italy (författare)
Ljung, KarinSwedish Univ Agr Sci, Sweden (författare)
Bonza, Maria CristinaUniv Milan, Italy (författare)
Stavrinidou, EleniLinköpings universitet,Laboratoriet för organisk elektronik,Tekniska fakulteten,Swedish Univ Agr Sci, Sweden,Wallenberg Wood Science Center(Swepub:liu)elest58 (författare)
Costa, AlexUniv Milan, Italy; Natl Res Council Italy CNR, Italy (författare)
Univ Milan, ItalyLaboratoriet för organisk elektronik (creator_code:org_t)

Av författaren/redakt...: Grenzi, Matteo; Buratti, Stefano; Parmagnani, Ambr ...; Abdel Aziz, Ilar ...; Bernacka Wojcik, ...; Resentini, Franc ...; visa fler...; Simura, Jan; Doccula, Fabrizi ...; Alfieri, Andrea; Luoni, Laura; Ljung, Karin; Bonza, Maria Cri ...; Stavrinidou, Ele ...; Costa, Alex; visa färre...

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