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Novel Pentameric Th...
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Åslund, AndreasLinköpings universitet,Organisk Kemi,Tekniska högskolan
(författare)
Novel Pentameric Thiophene Derivatives for in Vitro and in Vivo Optical Imaging of a Plethora of Protein Aggregates in Cerebral Amyloidoses
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
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2009-07-30
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American Chemical Society (ACS),2009
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-20420
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-20420URI
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https://doi.org/10.1021/cb900112vDOI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Molecular probes for selective Identification of protein aggregates are important to advance our understanding of the molecular pathogenesis underlying cerebral amyloidoses. Here we report the chemical design of pentameric thiophene derivatives, denoted luminescent conjugated oligothiophenes (LCOs), which could be used for real-time visualization of cerebral protein aggregates in transgenic mouse models of neurodegenerative diseases by multiphoton microscopy. One of the LCOs, p-FTAA, could be utilized for ex vivo spectral assignment of distinct prion deposits from two, mouse-adapted prion strains. p-FTAA also revealed a transient soluble pre-fibrillar non-thioflavinophilic A beta-assemblies during in vitro fibrillation of A beta peptides. In brain tissue samples, A beta deposits and neurofibrillary tangles (NFTs) were readily identified by a strong fluorescence from p-FTAA and the LCO staining showed complete co-localliation with conventional antibodies (6E10 and AT8). In addition, a patchy islet-like staining of individual A beta plaque was unveiled by the anti-oligomer A11 antibody during co-staining with p-FTAA. The major hallmarks of Alzheimers disease, namely, A beta aggregates versus NFTs, could also be distinguished because of distinct emission spectra from p-FTAA. Overall, we demonstrate that LCOs can be utilized as powerful practical research tools for studying protein aggregation diseases and facilitate the study of amyloid origin, evolution and maturation, A beta-tau interactions, and pathogenesis both ex vivo and in vivo.
Ämnesord och genrebeteckningar
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NATURAL SCIENCES
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NATURVETENSKAP
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Sigurdson, Christina JInstitute of Neuropathology, Department of Pathology, Universitätsspital Zürich, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland
(författare)
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Klingstedt, TheréseLinköpings universitet,Kemi,Tekniska fakulteten(Swepub:liu)thekl87
(författare)
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Grathwohl, StefanDepartment of Cellular Neurology, Hertie-Institute for Clinical Brain Research, University of Tuebingen, D-72076 Tuebingen, Germany
(författare)
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Bolmont, TristanDepartment of Cellular Neurology, Hertie-Institute for Clinical Brain Research, University of Tuebingen, D-72076 Tuebingen, Germany
(författare)
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Dickstein, Dara LDepartment of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
(författare)
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Glimsdal, EirikDepartment of Physics, Norwegian University of Science and Technology, N-7491 Trondheim, Norway
(författare)
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Prokop, StefanDepartment of Neuropathology, Charité-Universitätsmedizin Berlin, D-13353 Berlin, Germany
(författare)
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Lindgren, MikaelDepartment of Physics, Norwegian University of Science and Technology, N-7491 Trondheim, Norway
(författare)
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Konradsson, PeterLinköpings universitet,Organisk Kemi,Tekniska högskolan(Swepub:liu)petko14
(författare)
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Holtzman, David MDepartment of Neurology, Alzheimer’s Disease Research Center, Washington University, St. Louis, Missouri 63110, USA
(författare)
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Hof, Patrick RDepartment of Neuroscience, Mount Sinai School of Medicine, New York, New York 10029, USA
(författare)
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Heppner, Frank LDepartment of Neuropathology, Charité-Universitätsmedizin Berlin, D-13353 Berlin, Germany
(författare)
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Gandy, SamuelAlzheimer’s Disease Research Center, Mount Sinai School of Medicine, New York, New York 10029, USA
(författare)
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Jucker, MathiasDepartment of Cellular Neurology, Hertie-Institute for Clinical Brain Research, University of Tuebingen, D-72076 Tuebingen, Germany
(författare)
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Aguzzi, AdrianoInstitute of Neuropathology, Department of Pathology, Universitätsspital Zürich, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland
(författare)
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Hammarström, PerLinköpings universitet,Biokemi,Tekniska högskolan(Swepub:liu)perha81
(författare)
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Nilsson, PeterLinköpings universitet,Organisk Kemi,Tekniska högskolan(Swepub:liu)petni61
(författare)
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Linköpings universitetOrganisk Kemi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:ACS CHEMICAL BIOLOGY: American Chemical Society (ACS)4:8, s. 673-6841554-89291554-8937
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Åslund, Andreas
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Sigurdson, Chris ...
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Klingstedt, Ther ...
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Grathwohl, Stefa ...
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Bolmont, Tristan
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Dickstein, Dara ...
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visa fler...
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Glimsdal, Eirik
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Prokop, Stefan
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Lindgren, Mikael
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Konradsson, Pete ...
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Holtzman, David ...
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Hof, Patrick R
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Heppner, Frank L
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Gandy, Samuel
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Jucker, Mathias
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Aguzzi, Adriano
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Hammarström, Per
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Nilsson, Peter
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