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Interferon-gamma se...
Interferon-gamma secreted from peripheral blood mononuclear cells as a possible diagnostic marker for allergic contact dermatitis to gold
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- Christiansen Clifford, Jenny (författare)
- Linköpings universitet,Molekylär och immunologisk patologi,Hälsouniversitetet
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- Färm, Gunilla (författare)
- Department of Dermatology, University Hospital, 701 85 Örebro, Sweden
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- Eid-Forest, Ruth (författare)
- Department of Dermatology, University Hospital, 701 85 Örebro, Sweden
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- Anderson, Chris (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Dermatologi och venerologi,Hälsouniversitetet,Hudkliniken US
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- Cederbrant, Karin (författare)
- Linköpings universitet,Molekylär och immunologisk patologi,Hälsouniversitetet,Department of Molecular Toxicology, Safety Assessment, AstraZeneca, 151 85 Södertäje, Sweden
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- Hultman, Per (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Molekylär och immunologisk patologi,Hälsouniversitetet,Klinisk patologi och cytologi
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(creator_code:org_t)
- Wiley, 2006
- 2006
- Engelska.
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Ingår i: Contact Dermatitis. - : Wiley. - 0105-1873 .- 1600-0536. ; 55:2, s. 101-112
- Relaterad länk:
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http://urn.kb.se/res...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- 10% of patch-tested patients have a positive reaction to gold. Most lack clinical symptoms, but allergic contact dermatitis (ACD) to gold is increasing. In this study, 77 dermatological outpatients were divided into 3 groups depending on epicutaneous patch test outcomes: a group positive to gold (EPI+), a group negative to gold (EPI-), and a group with irritant reactions to gold (EPI-IR). Lymphocytes were stimulated in vitro with gold sodium thiosulfate. Proliferation was assessed using the lymphocyte transformation test (LTT), and cytokine secretion was assessed using a multibead array (Luminex; Linco Research Inc., St. Charles, MO, USA), in order to evaluate whether an in vitro method with high diagnostic accuracy could be devised. The EPI+ group showed a significantly increased secretion of interferon (IFN)-gamma, interleukin (IL)-2, and IL-13 and also showed a significantly higher stimulation indexes for LTT, compared to the other 2 subject groups. Sensitivity and specificity were calculated for all methods individually and combined, but IFN-gamma assessment alone was the most accurate method for identifying ACD to gold, with sensitivity and specificity of 81.8% and 82.1%, respectively. This method also identified 87.5% of the EPI-IR subjects as non-allergic. Therefore, assessment of secretion of IFN-gamma should be a valuable complement to patch test for diagnosing gold allergy.
Nyckelord
- Allergic contact dermatitis
- cytokines
- gold
- interferon-γ
- lymphocyte transformation test
- multibead assay
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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