Search: WFRF:(Lindahl Tomas 1954 )
> (1999) >
Surface plasmon res...
Surface plasmon resonance (SPR) analysis of coagulation in whole blood with application in prothrombin time assay
-
- Hansson, Kenny, 1972- (author)
- Linköpings universitet,Klinisk kemi,Hälsouniversitetet
-
- Vikinge, T. P. (author)
- Linköpings universitet,Tillämpad Fysik,Hälsouniversitetet
-
- Rånby, M. (author)
- Linköpings universitet,Klinisk kemi,Hälsouniversitetet
-
show more...
-
- Tengvall, Pentti, 1951- (author)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
-
- Lundström, Ingemar, 1941- (author)
- Linköpings universitet,Tillämpad Fysik,Tekniska högskolan
-
- Johansen, Knut (author)
- Linköpings universitet,Tillämpad Fysik,Hälsouniversitetet
-
- Lindahl, Tomas, 1954- (author)
- Linköpings universitet,Klinisk kemi,Hälsouniversitetet
-
show less...
-
(creator_code:org_t)
- 1999
- 1999
- English.
-
In: Biosensors & bioelectronics. - 0956-5663 .- 1873-4235. ; 14:8-9, s. 671-682
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- It is previously shown that surface plasmon resonance (SPR) can be used to study blood plasma coagulation. This work explores the use of this technique for the analysis of tissue factor induced coagulation, i.e. prothrombin time (PT) analysis, of whole blood and plasma. The reference method was nephelometry. The prothrombin time analysis by SPR was performed by mixing two volumes of blood/plasma, one volume of thromboplastin, and one volume of CaCl2 solution directly on a sensor surface. The measurements show good agreement between nephelometry and SPR plasma analysis and also between SPR plasma and whole blood analysis. The effect of anticoagulant treatment on the clotting times was significant both quantitatively and qualitatively. The impact on the SPR signal of different physiological events in the coagulation process is discussed, and tentative interpretations of the sensorgram features are given. The major advantage of the SPR method compared to nephelometry is the possibility to perform analysis on whole blood instead of plasma. In conclusion, SPR is a promising method for whole blood coagulation analysis.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database