Quantitative relationships between pigment-related mRNA and biochemical melanoma markers in melanoma cell lines

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Johansson, Malin,1972-Linköpings universitet,Klinisk kemi,Hälsouniversitetet (författare)

Quantitative relationships between pigment-related mRNA and biochemical melanoma markers in melanoma cell lines

Artikel/kapitelEngelska2002

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Ovid Technologies (Wolters Kluwer Health),2002
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LIBRIS-ID:oai:DiVA.org:liu-25118
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-25118URI
https://doi.org/10.1097/00008390-200206000-00002DOI

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Språk:engelska
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The use of reverse transcription polymerase chain reaction (RT-PCR) analysis of melanoma-specific transcripts for the identification of circulating melanoma cells has shown very variable results in different studies on melanoma patients. We have therefore developed quantitative methods to study both analytical and biological variations as possible causes of this phenomenon. Pigment-related and S-100β transcripts were quantified in 12 different melanoma cell lines and related to the amounts of 5-S-cysteinyldopa, pigment and S-100B protein. A real-time PCR method was used and the results were expressed as absolute number of transcripts per cell. Tyrosinase, tyrosinase-related protein (TRP)-1, TRP-2 and MART-1/Melan-A mRNA varied from undetectable (< 10-4 transcripts/cell) to 103 transcripts/cell, i.e. by a factor > 107 in the different cell lines. S-100β mRNA varied from 2.8 to 165 transcripts/cell, i.e. by a factor of 60. Tyrosinase, TRP-1 and TRP-2 mRNA correlated significantly with the amount of 5-S-cysteinyldopa, an intermediate pigment metabolite (P < 0.001, P < 0.001 and P < 0.01, respectively). The amount of S-100β mRNA correlated significantly with the amount of S-100B protein (P < 0.001). No cross-correlations were seen between the pigment-related and S-100-related analytes. We conclude that one reason behind the negative results of RT-PCR measurement of pigment-related mRNA may be that these transcripts are not always expressed in the particular cells present in the patient's blood. Furthermore, variation in the expression of the order of 107 must have great impact on the diagnostic sensitivity. Measurement of S-100β mRNA would be more sensitive, but the use of this transcript is hampered by its presence in the blood cells. © 2002 Lippincott Williams & Wilkins.

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MEDICINE
MEDICIN

Biuppslag (personer, institutioner, konferenser, titlar ...)

Takasaki, A.Linköpings universitet,Klinisk kemi,Hälsouniversitetet (författare)
Lenner, Liselotte,1954-Linköpings universitet,Klinisk kemi,Hälsouniversitetet(Swepub:liu)lisle27 (författare)
Årstrand, Kerstin,1946-Linköpings universitet,Klinisk kemi,Hälsouniversitetet(Swepub:liu)kerar67 (författare)
Kågedal, Bertil,1943-Linköpings universitet,Klinisk kemi,Hälsouniversitetet(Swepub:liu)berka70 (författare)
Linköpings universitetKlinisk kemi (creator_code:org_t)

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Ingår i:Melanoma research: Ovid Technologies (Wolters Kluwer Health)12:3, s. 193-2000960-89311473-5636

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Av författaren/redakt...: Johansson, Malin ...; Takasaki, A.; Lenner, Liselott ...; Årstrand, Kersti ...; Kågedal, Bertil, ...

Artiklar i publikationen: Melanoma researc ...

Av lärosätet: Linköpings universitet

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