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A Lectin Immunosens...
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Liljeblad, MathiasLinköpings universitet,Klinisk kemi,Hälsouniversitetet
(författare)
A Lectin Immunosensor Technique for Determination of α1-Acid Glycoprotein Fucosylation
- Artikel/kapitelEngelska2001
Förlag, utgivningsår, omfång ...
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Elsevier BV,2001
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-25224
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-25224URI
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https://doi.org/10.1006/abio.2000.4905DOI
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The fucosylation of α1-acid glycoprotein (AGP), an acute-phase protein, is known to change in association with inflammatory diseases. Thus, fucosylation of AGP could be a potential diagnostic or prognostic marker. The change in fucosylation has previously been investigated using crossed affinoimmunoelectrophoresis, high-pH anion-exchange chromatography, and lectin ELISA. This study describes a surface plasmon resonance-based affinity biosensor assay for quantification of the fucosylation of AGP. Diluted EDTA plasma or serum was injected directly in a BIACORE 2000 biosensor. AGP was captured on the sensor surface using immobilized antibodies and a fucose-binding lectin from Aleuria aurentia was then used for the detection of fucosylation. The feature of the biosensor makes it possible to determine both the amount of bound AGP and the amount of bound lectin. Using a calibration curve it was possible to obtain a fucosylation ratio that was independent of AGP concentration. The assay was validated against a lectin ELISA and used to follow inflammation in patients with severe burns.
Ämnesord och genrebeteckningar
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a1-acid glycoprotein
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Aleuria aurentia lectin
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Fucose
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Glycosylation
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Surface plasmon resonance
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MEDICINE
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MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Rydén, IngvarDepartment of Clinical Chemistry, Kalmar County Hospital, Kalmar, Sweden
(författare)
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Ohlson, StenDivision of Biochemistry/Biotechnology, Department of Chemistry and Biomedical Sciences, University of Kalmar, Kalmar, Sweden
(författare)
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Lundblad, Arne,1937-Linköpings universitet,Klinisk kemi,Hälsouniversitetet(Swepub:liu)arnlu37
(författare)
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Påhlsson, Peter,1962-Linköpings universitet,Klinisk kemi,Hälsouniversitetet(Swepub:liu)petpa61
(författare)
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Linköpings universitetKlinisk kemi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Analytical Biochemistry: Elsevier BV288:2, s. 216-2240003-26971096-0309
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