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PCR Identification ...
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Orvelid, PaulaDepartment of Clinical Microbiology and Immunology, Örebro Medical Center Hospital, Örebro, Sweden
(författare)
PCR Identification of the Group A Neisseria Meningitidis Gene in Cerebrospinal Fluid
- Artikel/kapitelEngelska1999
Förlag, utgivningsår, omfång ...
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2009-07-08
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Informa UK Limited,1999
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-26071
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-26071URI
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https://doi.org/10.1080/00365549950164003DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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The aim of this study was to develop a PCR method for direct identification of Neisseria meningitidis serogroup A in cerebrospinal fluid. The assay makes use of unique sites within the gene cassette responsible for expression of the (α1→6)-linked N-acetyl-D-mannosamine-1-phosphate serogroup A capsule. A total of 67 different N. meningitidis strains and 12 clinical samples of CSF, culture positive for N. meningitidis, were examined. All the strains and samples of N. meningitidis serogroup A were correctly identified by an amplified PCR product of 519 bp. The PCR method for identification is specific for the group A gene of N. meningitidis. The assay may contribute to reducing recurrent, devastating epidemics of meningococcal infection by providing a diagnostic tool for grouping in developing countries where problems with false negative cultures are common and vaccination against serogroup A meningococci may be required.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Bäckman, AndersDepartment of Clinical Microbiology and Immunology, Örebro Medical Center Hospital, Örebro, Sweden
(författare)
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Olcén, Per,1943-Department of Clinical Microbiology and Immunology, Örebro Medical Center Hospital, Örebro, Sweden(Swepub:liu)perol69
(författare)
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Department of Clinical Microbiology and Immunology, Örebro Medical Center Hospital, Örebro, Sweden
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Scandinavian Journal of Infectious Diseases: Informa UK Limited31:5, s. 481-4830036-55481651-1980
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