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Increased IFN-? res...
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Svenvik, MÖstergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Klinisk immunologi,Klinisk immunologi och transfusionsmedicin
(author)
Increased IFN-? response to transplantation antigens measured by cytokine MLC : Indications for a bi-phasic response pattern
- Article/chapterEnglish2003
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Numbers
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LIBRIS-ID:oai:DiVA.org:liu-26418
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-26418URI
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https://doi.org/10.1016/S0966-3274(02)00146-6DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The immunological reactivity to major histocompatibility transplantation antigens (MHC) is important in clinical manifestations such as graft rejection and graft-vs.-host disease. To evaluate the allogeneic cytokine response pattern we used a newly developed cytokine mixed leukocyte culture (MLC) technique. Blood mononuclear cells from healthy women (n=6) were exposed to cells from another person and to pooled cells from 28 blood donors (MHC-pool). The secretion kinetics of IL-4 and IFN-? from the responder cells was analysed by ELISPOT. We found a higher IFN-? response to the MHC-pool compared with the IL-4 secretion. Both the total secretion of IFN-? for 7 days and the median value of IFN-? in each individual was increased compared with IL-4. The IFN-? response showed a bi-phasic pattern with the major peak on day 6-7. Our results indicate that allo-responses are mainly Thl-like responses, displaying a bi-phasic pattern. This knowledge may be useful, and the methods suitable, in the studies of allo-responses in transplantation and pregnancy.
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Ekerfelt, Christina,1957-Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Klinisk immunologi,Klinisk immunologi och transfusionsmedicin(Swepub:liu)chrek55
(author)
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Linköpings universitetHälsouniversitetet
(creator_code:org_t)
Related titles
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In:Transplant Immunology11:1, s. 101-1050966-32741878-5492
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