Search: WFRF:(Svensson Samuel 1962 )
> (2000-2004) >
Regulation of melan...
Regulation of melanosome movement by MAP kinase
-
- Andersson, Tony, 1973- (author)
- Linköpings universitet,Farmakologi,Hälsouniversitetet
-
- Svensson, Samuel, 1962- (author)
- Linköpings universitet,Farmakologi,Hälsouniversitetet
-
- Karlsson, Annika M., 1972- (author)
- Linköpings universitet,Farmakologi,Hälsouniversitetet
-
(creator_code:org_t)
- Wiley, 2003
- 2003
- English.
-
In: Pigment Cell Research. - : Wiley. - 0893-5785 .- 1600-0749. ; 16:3, s. 215-221
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Our objectives were to further characterize the signaling pathways in melatonin-induced aggregation in Xenopus melanophores, specifically to investigate a possible role of mitogen-activated protein kinase (MAPK). By Western blotting we found that melatonin activates MAPK, which precedes melanosome aggregation measured in a microplate reader. Activation of MAPK, tyrosine phosphorylation of a previously described 280-kDa protein, and melanosome aggregation are sensitive to PD98059, a selective inhibitor of MAPK kinase. The MAPK activation is also decreased by the adenylate cyclase stimulant forskolin. In summary, we found that MAPK is activated during melatonin-induced melanosome aggregation. Activation was decreased by an inhibitor of MAPK kinase, and by forskolin. In addition to inhibition of cyclic adenosine 3′,5′-monophosphate (cAMP), reduction in protein kinase A activity (PKA), and activation of protein phosphatase 2A, we suggest that melatonin receptors activate the MAPK cascade and tyrosine phosphorylation of the 280-kDa protein. Although the cAMP/PKA signaling pathway is the most prominent, our data suggest that simultaneous activation of the MAPK cascade is of importance to obtain a completely aggregated state. This new regulatory mechanism of organelle transport by the MAPK cascade might be important in other eukaryotic cells.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database