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Molecular cloning o...
Molecular cloning of an unusual bicistronic cholecystokinin receptor mRNA expressed in chicken brain : a structural and functional expression study
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- Nilsson, Isabelle, 1971- (författare)
- Linköpings universitet,Östergötlands Läns Landsting,LMÖ - Laboratoriemedicin i Östergötland,Farmakologi,Hälsouniversitetet
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- Svensson, Samuel, 1962- (författare)
- Linköpings universitet,Farmakologi,Hälsouniversitetet
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- Monstein, Hans-Jürg (författare)
- Linköpings universitet,Östergötlands Läns Landsting,LMÖ - Laboratoriemedicin i Östergötland,Institutionen för biomedicin och kirurgi,Hälsouniversitetet
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(creator_code:org_t)
- 2003
- 2003
- Engelska.
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Ingår i: Regulatory Peptides. - 0167-0115 .- 1873-1686. ; 114:1, s. 37-43
- Relaterad länk:
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https://urn.kb.se/re...
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visa fler...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- This report describes the molecular cloning and pharmacological characterization of a transiently expressed chicken brain cholecystokinin receptor (CCK-CHR) in COS-7 cells. A polymerase chain reaction (PCR)-based cloning strategy was applied using: (1) an initial PCR with deoxyinosine-containing primers designed to target conserved regions in CCK receptors, followed by (2) rapid amplification of cDNA ends (RACE), and (3) full-length PCR of the CCK-CHR cDNA. The full-length cloned bicistronic CCK-CHR cDNA contained a short upstream open reading frame (uORF) coding for a putative six-amino-acid-long peptide of unknown function, followed by a long open reading frame (lORF) encoding the 436-amino-acid-long CCK-CHR receptor protein. At the amino acid level, the CCK-CHR shared ∼50% homology with mammalian and Xenopus laevis CCK receptors. The pharmacological profile of CCK-CHR resembled that of CCK-B receptors using agonists (CCK-8, CCK-4, gastrin-17), whereas CCK-CHR showed higher affinity for the CCK-A receptor antagonist, devazepide, than for the CCK-B receptor antagonist, l-365,260. To the best of our knowledge, this is the first description and functional expression study of a cloned chicken CCK receptor cDNA.
Nyckelord
- MEDICINE
- MEDICIN
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- ref (ämneskategori)
- art (ämneskategori)
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