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Molecular identific...
Molecular identification of Helicobacter DNA present in human colorectal adenocarcinomas by 16S rDNA PCR amplification and pyrosequencing analysis
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- Grahn, Niclas (författare)
- Molecular Biology Laboratory - LMC, University Hospital, Linköping, Sweden; Division of Cell Biology, Department of Biomedicine and Surgery, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Laboratory of Human Molecular Genetics, Faculty of Medicine of Sfax, Tunisia
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- Hmani-Aifa, Mounira (författare)
- Molecular Biology Laboratory - LMC, University Hospital, Linköping, Sweden; Division of Cell Biology, Department of Biomedicine and Surgery, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Laboratory of Human Molecular Genetics, Faculty of Medicine of Sfax, Tunisia
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- Fransén, Karin, 1973- (författare)
- Linköpings universitet,Hälsouniversitetet,Avdelningen för medicinsk cellbiologi,Molecular Biology Laboratory - LMC, University Hospital, Linköping, Sweden; Division of Cell Biology, Department of Biomedicine and Surgery, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Laboratory of Human Molecular Genetics, Faculty of Medicine of Sfax, Tunisia
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- Söderkvist, Peter, 1953- (författare)
- Linköpings universitet,Hälsouniversitetet,Avdelningen för medicinsk cellbiologi,Molecular Biology Laboratory - LMC, University Hospital, Linköping, Sweden; Division of Cell Biology, Department of Biomedicine and Surgery, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Laboratory of Human Molecular Genetics, Faculty of Medicine of Sfax, Tunisia
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- Monstein, Hans-Jurg, 1946- (författare)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Avdelningen för medicinsk cellbiologi,Molekylärbiologiska tekniklaboratoriet,Molecular Biology Laboratory - LMC, University Hospital, Linköping, Sweden; Division of Cell Biology, Department of Biomedicine and Surgery, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Laboratory of Human Molecular Genetics, Faculty of Medicine of Sfax, Tunisia
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(creator_code:org_t)
- Microbiology Society, 2005
- 2005
- Engelska.
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Ingår i: Journal of Medical Microbiology. - : Microbiology Society. - 0022-2615 .- 1473-5644. ; 54:11, s. 1031-1035
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Seroepidemiological studies have indicated that Helicobacter pylori infection might be a possible risk factor for colorectal adenocarcinoma (CRC) development. However, limited information is available as to whether or not Helicobacter species are present in CRC tissues. In this study the presence of Helicobacter DNA in 77 CRC biopsies was investigated by means of a Helicobacter species-specific 16S rDNA PCR assay and real-time DNA pyrosequencing of the 16S rDNA variable V3 region. Pyrosequencing revealed the presence of Helicobacter DNA sequences in 21 of 77 biopsy specimens (27%). 16S rDNA sequences corresponding to H. pylori 26695 and H. pylori J99 were most commonly found. Intriguingly, one sequence belonged to Helicobacter mustelae, previously identified in ferrets. No significant correlations were found in the prevalence of Helicobacter DNA between colon and rectum tumour biopsies (P = 0.815), nor between Dukes' classes A/B and C/D (P = 0.262). 16S rDNA PCR amplification combined with pyrosequencing analysis of 16S rDNA variable V3 regions provides a powerful molecular tool to identify Helicobacter species in human biopsy specimens. © 2005 SGM.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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