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Interlaboratory Comparison of PCR-Based Identification and Genogrouping of Neisseria meningitidis

Taha, Muhamed-Kheir (författare)
Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France
Alonso, Jean-Michel (författare)
Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France
Cafferkey, Mary (författare)
Irish Meningococcal and Meningitis Reference Laboratory, The Children's University Hospital, Dublin, Ireland
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Caugant, Dominique A. (författare)
WHO Collaborating Center for Reference and Research on Meningococci, Division of Infectious Disease Control, Norwegian Institute of Public Health and Institute of Oral Biology, University of Oslo, Oslo, Norway
Clarke, Stuart C. (författare)
Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital and Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow
Diggle, Mathew A. (författare)
Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital, Glasgow
Fox, Andrew (författare)
Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom
Frosch, Matthias (författare)
Institute for Hygiene and Microbiology, National Reference Center for Meningococci, University of Würzburg, Germany
Gray, Stephen J. (författare)
Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom
Guiver, Malcolm (författare)
Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom
Heuberger, Sigrid (författare)
National Reference Centre for Meningococci, Austrian Agency for Health and Food Safety, Graz, Austria
Kalmusova, Jitka (författare)
National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Prague, Czech Republic
Kesanopoulos, Konstantinos (författare)
National Meningococcal Reference Laboratory, NIPH, Athens, Greece
Klem, Anne-Marie (författare)
WHO Collaborating Center for Reference and Research on Meningococci, Division of Infectious Disease Control, Norwegian Institute of Public Health, Oslo, Norway
Kriz, Paula (författare)
National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Prague, Czech Republic
Marsh, John (författare)
Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom
Mölling, Paula (författare)
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, University Hospital, Örebro, Sweden
Murphy, Karen (författare)
Irish Meningococcal and Meningitis Reference Laboratory, The Children's University Hospital, Dublin, Ireland
Olcén, Per, 1943- (författare)
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, University Hospital, Örebro, Sweden
Sanou, Oumar (författare)
Centre Muraz, Bobo Dioulasso, Burkina Faso
Tzanakaki, Georgina (författare)
National Meningococcal Reference Laboratory, NIPH, Athens, Greece
Vogel, Ulrich (författare)
Institute for Hygiene and Microbiology, National Reference Center for Meningococci, University of Würzburg, Germany
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 (creator_code:org_t)
2005
2005
Engelska.
Ingår i: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 43:1, s. 144-149
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.

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MEDICIN

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