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Importin beta :
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Ola, Thomas OQueen Mary College, London, UK
(author)
Importin beta : A novel autoantigen in human autoimmunity identified by screening random peptide libraries on phage
- Article/chapterEnglish2006
Publisher, publication year, extent ...
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Elsevier BV,2006
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-34824
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-34824URI
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https://doi.org/10.1016/j.jaut.2006.01.003DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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By screening random peptide libraries (RPLs) with sera of Type 1 diabetes (T1D) patients, we previously identified 5 disease-specific 'mimotopes' displayed on phages (phagotopes). We already characterised 1 phagotope (CH1p), as an epitope of human osteopontin, an autoantigen expressed within the somatostatin cells of human islets. In this paper, we report the characterization of the second phagotope, 195Dyn, by immunohistochemistry, Western Blotting and screening of a human islet cDNA library using rabbit anti-195Dyn antibodies. The 195Dyn mimotope was detected in human islets. The screening of a λgt11 cDNA library from human islets has identified a clone, which corresponded to human importin beta. ELISA detected autoantibodies against this protein in sera of around 60% of TD1 patients and in 30% of patients affected by other autoimmune diseases. In summary, RPLs technology proved again successful in identifying another novel autoantigen (importin beta), whose significance in the autoimmune process remains to be fully elucidated. © 2006 Elsevier Ltd. All rights reserved.
Subject headings and genre
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autoantigens
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autoimmunity
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peptide libraries
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MEDICINE
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MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
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Biro, Paul AQueen Mary College, London, UK
(author)
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Hawa, Mohammed IQueen Mary College, London, UK
(author)
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Ludvigsson, Johnny,1943-Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Pediatrik,Barn- och ungdomskliniken i Linköping(Swepub:liu)johlu29
(author)
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Locatelli, MatiaOspedale Pediatrico Bambino Gesu, Rome, Italy
(author)
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Puglisi, Maria AOspedale Pediatrico Babino Gesu, Rome, Italy
(author)
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Bottazzo, Gian FrancoOspedale Pediatrico Babino Gesu, Rome, Italy
(author)
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Fierabracci, AlessandraOspedale Pediatrico Babino Gesu, Rome, Italy
(author)
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Queen Mary College, London, UKHälsouniversitetet
(creator_code:org_t)
Related titles
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In:Journal of Autoimmunity: Elsevier BV26:3, s. 197-2070896-84111095-9157
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