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Decreased in vitro ...
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Skarsvik, Susanne,1978-Linköpings universitet,Pediatrik,Hälsouniversitetet
(författare)
Decreased in vitro type 1 immune response against coxsackie virus B4 in children with type 1 diabetes
- Artikel/kapitelEngelska2006
Förlag, utgivningsår, omfång ...
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American Diabetes Association,2006
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-34899
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-34899URI
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https://doi.org/10.2337/diabetes.55.04.06.db05-0630DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Enteroviruses, particularly Coxsackie virus B4 (CVB4), are considered to be involved in the pathogenesis of type 1 diabetes. We wanted to compare the characteristics of T-cell immune response to CVB4 in children with type 1 diabetes and healthy children with and without HLA risk-associated haplotypes (HLA-DR3-DQ2 or HLA-DR4-DQ8) for type 1 diabetes. Peripheral blood mononuclear cells (PBMCs) were isolated and cultured with CVB4 and analyzed for cytokine and chemokine receptors by flow cytometry and for expression of transcription factors Tbet and GATA-3 by RT-PCR and Western blot. Culture supernatants were analyzed for secretion of γ-interferon (IFN-γ). In children with type 1 diabetes, a decreased percentage of T-cells expressed CCR2, CXCR6, interleukin (IL)-18R, and IL-12Rβ2-chain after in vitro stimulation with CVB4 in comparison with healthy children with or without HLA risk genotype. Moreover, we found that children with type 1 diabetes had decreased IFN-γ secretion and expression of Tbet, both on mRNA and protein level, in CVB4-stimulated PBMCs. Accordingly, children with type 1 diabetes show an impaired type 1 immune response against CVB4 compared with healthy children. This may lead to a delayed clearance of the virus and, at least partly, explain why children with type 1 diabetes may be more prone to CVB4 infections and related complications, such as β-cell damage.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Puranen, JuliaLinköpings universitet,Pediatrik,Hälsouniversitetet
(författare)
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Honkanen, JarnoLaboratory for Immunobiology, Department of Viral Diseases and Immunology, National Public Health Institute, Helsinki, Finland
(författare)
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Roivainen, MerjaEnterovirus Laboratory, Department of Viral Diseases and Immunology, National Public Health Institute, Helsinki, Finland
(författare)
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Ilonen, JormaTurku Immunology Centre and Department of Virology, University of Turku, Turku, Finland
(författare)
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Holmberg, Hanna,1975-Linköpings universitet,Pediatrik,Hälsouniversitetet(Swepub:liu)hanho33
(författare)
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Ludvigsson, Johnny,1943-Linköpings universitet,Pediatrik,Hälsouniversitetet(Swepub:liu)johlu29
(författare)
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Vaarala, Outi,1962-Linköpings universitet,Pediatrik,Hälsouniversitetet(Swepub:liu)outva41
(författare)
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Linköpings universitetPediatrik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Diabetes: American Diabetes Association55:4, s. 996-10030012-17971939-327X
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Diabetes
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Skarsvik, Susann ...
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Puranen, Julia
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Honkanen, Jarno
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Roivainen, Merja
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Ilonen, Jorma
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Holmberg, Hanna, ...
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Ludvigsson, John ...
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Vaarala, Outi, 1 ...
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- Artiklar i publikationen
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Diabetes
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Linköpings universitet