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Role of the actin c...
Role of the actin cytoskeleton during respiratory burst in chemoattractant-stimulated neutrophils
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- Bengtsson, Torbjörn, 1955- (author)
- Linköpings universitet,Hälsouniversitetet,Farmakologi
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- Orselius, Kristina, 1954- (author)
- Linköpings universitet,Hälsouniversitetet,Medicinsk mikrobiologi
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- Wetterö, Jonas, 1972- (author)
- Linköpings universitet,Hälsouniversitetet,Reumatologi
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(creator_code:org_t)
- Wiley, 2006
- 2006
- English.
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In: Cell Biology International. - : Wiley. - 1065-6995 .- 1095-8355. ; 30:2, s. 154-163
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Abstract
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- The aim of this study was to clarify the role of the actin cytoskeleton during chemotactic peptide fMet-Leu-Phe (fMLF)-stimulated respiratory burst in human neutrophil granulocytes. Reactive oxygen species (ROS) was measured as luminol-amplified chemiluminescence (CL) and F-actin content as bodipy phallacidin fluorescence in neutrophils treated with latrunculin B or jasplakinolide, an inhibitor and activator of actin polymerization, respectively. Latrunculin B markedly decreased, whereas jasplakinolide increased, the F-actin content in neutrophils, unstimulated or stimulated with fMLF. Latrunculin B enhanced the fMLF-triggered ROS-production more than tenfold. Jasplakinolide initially inhibited the fMLF-induced CL-response, however, caused a potent second sustained phase (>400% of control). Both actin drugs triggered a substantial CL-response when added 5-25 min after fMLF. This was also valid for chemotactic doses of fMLF, where latrunculin B and jasplakinolide amplified the ROS-production 5-10 times. By using specific signal transduction inhibitors, we found that the NADPH oxidase activation triggered by destabilization of the actin cytoskeleton occurs downstream of phospholipase C and protein kinase C but is mediated by Rho GTPases and tyrosine phosphorylation. In conclusion, rearrangements of the actin cytoskeleton are a prerequisite in connecting ligand/receptor activation, generation of second messengers and assembly of the NADPH oxidase in neutrophil granulocytes. © 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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