WFRF:(Bengtsson Torbjörn 1955 )
Sökning: WFRF:(Bengtsson Torbjörn 1955 ) > Role of the actin c...
- Bengtsson, Torbjörn,1955-Linköpings universitet,Hälsouniversitetet,Farmakologi (författare)
Role of the actin cytoskeleton during respiratory burst in chemoattractant-stimulated neutrophils
- Artikel/kapitelEngelska2006
Förlag, utgivningsår, omfång ...
- Wiley,2006
- electronicrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:liu-35148
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-35148URI
- https://doi.org/10.1016/j.cellbi.2005.10.017DOI
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- Språk:engelska
- Sammanfattning på:engelska
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- The aim of this study was to clarify the role of the actin cytoskeleton during chemotactic peptide fMet-Leu-Phe (fMLF)-stimulated respiratory burst in human neutrophil granulocytes. Reactive oxygen species (ROS) was measured as luminol-amplified chemiluminescence (CL) and F-actin content as bodipy phallacidin fluorescence in neutrophils treated with latrunculin B or jasplakinolide, an inhibitor and activator of actin polymerization, respectively. Latrunculin B markedly decreased, whereas jasplakinolide increased, the F-actin content in neutrophils, unstimulated or stimulated with fMLF. Latrunculin B enhanced the fMLF-triggered ROS-production more than tenfold. Jasplakinolide initially inhibited the fMLF-induced CL-response, however, caused a potent second sustained phase (>400% of control). Both actin drugs triggered a substantial CL-response when added 5-25 min after fMLF. This was also valid for chemotactic doses of fMLF, where latrunculin B and jasplakinolide amplified the ROS-production 5-10 times. By using specific signal transduction inhibitors, we found that the NADPH oxidase activation triggered by destabilization of the actin cytoskeleton occurs downstream of phospholipase C and protein kinase C but is mediated by Rho GTPases and tyrosine phosphorylation. In conclusion, rearrangements of the actin cytoskeleton are a prerequisite in connecting ligand/receptor activation, generation of second messengers and assembly of the NADPH oxidase in neutrophil granulocytes. © 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
Ämnesord och genrebeteckningar
- MEDICINE
- MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Orselius, Kristina,1954-Linköpings universitet,Hälsouniversitetet,Medicinsk mikrobiologi(Swepub:liu)krior09 (författare)
- Wetterö, Jonas,1972-Linköpings universitet,Hälsouniversitetet,Reumatologi(Swepub:liu)jonwe26 (författare)
- Linköpings universitetHälsouniversitetet (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Cell Biology International: Wiley30:2, s. 154-1631065-69951095-8355
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- Cell Biology International (Sök värdpublikationen i LIBRIS)
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- Linköpings universitet
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