Sökning: WFRF:(Andersson Lars Erik 1942 ) >
CP-115,953 stimulat...
CP-115,953 stimulates cytokine production by lymphocytes
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- Riesbeck, K. (författare)
- Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups,Skåne University Hospital
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- Forsgren, A. (författare)
- Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups,Skåne University Hospital
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(creator_code:org_t)
- 1995
- 1995
- Engelska.
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Ingår i: Antimicrobial Agents and Chemotherapy. - 0066-4804. ; 39:2, s. 476-483
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The cytotoxic quinolone CP-115,953 specifically exerts its inhibitory effect upon eukaryotic topoisomerase II. CP-115,953 stimulates DNA cleavage mediated by topoisomerase II with a potency approximately 600 times greater than that of ciprofloxacin, a quinolone antibacterial agent that currently is in clinical use. Because ciprofloxacin has been reported to strongly enhance interleukin-2 production, we considered it important to study the effect of CP-115,953 on interleukin-2 and gamma interferon (IFN-γ) mRNA and protein expression in mitogen-stimulated human peripheral blood lymphocytes. For comparison, novobiocin and the antineoplastic drug etoposide were also included in the study. CP-115,953 (25 μM) enhanced interleukin-2 mRNA levels up to 8-fold and IFN-γ mRNA concentrations up to 6.5-fold. In contrast, ciprofloxacin (282 μM) induced mRNAs for interleukin-2 and IFN-γ up to 20- fold and 7.8-fold, respectively. However, CP-115,953 showed more prolonged kinetics of IFN-γ mRNA production than ciprofloxacin. At high concentrations (≥141 μM), ciprofloxacin was a greater inducer of interleukin-2 production and exhibited a higher level of stimulatory action than CP-115,953 on IFN-γ synthesis. At low concentrations, however, CP-115,953 (≤25 μM) was more potent than ciprofloxacin in inducing interleukin-2 and IFN-γ synthesis. Etoposide or novobiocin did not influence cytokine mRNA expression. Thus, among the topoisomerase II inhibitors tested, fluoroquinolones are unique in stimulating cytokine synthesis in lymphocyte cultures.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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