Search: WFRF:(Wakamatsu Kazumasa) >
Determination of eu...
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Wakamatsu, Kazumasa
(author)
Determination of eumelanin in human urine
- Article/chapterEnglish2006
Publisher, publication year, extent ...
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Wiley,2006
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-35866
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-35866URI
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https://doi.org/10.1111/j.1600-0749.2006.00296.xDOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Normal and malignant melanocytes produce melanins and melanin-related metabolites, most of which are retained in the cells but some are secreted into the blood and then excreted in the urine. In this study, we developed a method to measure levels of eumelanin in urine samples and evaluated its clinical significance in comparison with the melanin-related metabolites 6-hydroxy-5-methoxyindole-2-carboxylic acid (6H5MI2C) and 5-S-cysteinyldopa (5-S-CD), and with pheomelanin, measured after degradation as 4-amino-3-hydroxyphenylalanine (4-AHP). The method is based on the production of pyrrole-2,3,5-tricarboxylic acid (PTCA) on permanganate oxidation of eumelanin, followed by quantification by liquid chromatography. For 118 urine samples from 10 control subjects, mean urinary excretions of PTCA, 6H5MI2C, 5-S-CD and 4-AHP were 19, 67, 37 and 59 μmol/mol creatinine respectively. In melanoma patients (n = 45), the mean urinary excretions of PTCA, 6H5MI2C, 5-S-CD, and 4-AHP were 91, 926, 4070 and 3530 μmol/mol creatinine respectively. Median level of PTCA in melanoma patients was elevated 2.1-fold compared with control subjects. The degrees of elevation for 6H5MI2C, 5-S-CD, and 4-AHP were 1.8-, 22- and 6.2-fold respectively. Thus, although urinary PTCA is of little clinical value in following the progression of melanoma, urinary 4-AHP appears to be of considerable value in this respect. © 2006 Blackwell Munksgaard.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Takasaki, Akihiko
(author)
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Kågedal, Bertil,1943-Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Avdelningen för klinisk kemi,Klinisk kemi(Swepub:liu)berka70
(author)
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Kageshita, Toshiro
(author)
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Ito, Shosuke
(author)
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Linköpings universitetHälsouniversitetet
(creator_code:org_t)
Related titles
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In:Pigment Cell Research: Wiley19:2, s. 163-1690893-57851600-0749
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