Sökning: WFRF:(Jenmalm Maria 1971 ) >
Expression of and r...
Expression of and responses to CD2 and CD3 in 18-month-old children with and without atopic dermatitis
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- Jenmalm, Maria, 1971- (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
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- Aniansson Zdolsek, Helena, 1961- (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
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- Holt, Patrocl G. (författare)
- TVW Telethon Institute for Child Health Research, Division of Cell Biology, Perth, Western Australia
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- Björkstén, Bengt (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
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(creator_code:org_t)
- 2008-10-09
- 2000
- Engelska.
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Ingår i: Pediatric Allergy and Immunology. - : Wiley. - 0905-6157 .- 1399-3038. ; 11:3, s. 175-182
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We hypothesize that atopy is associated with a reduced T-cell function early in life and an imbalance in cytokine production. The purpose of this study was to investigate the expression of and responses to CD2 and CD3 in children who did or did not develop atopic dermatitis early in life. The expression of CD2 and CD3 was analyzed by flow cytometry, and proliferation of CD2 and CD3 was studied by 3H-thymidine incorporation in phytohaemagglutinin (PHA)- and anti-CD3-stimulated peripheral blood mononuclear cells (PBMC) of 18-month-old children, 25 with and 29 without atopic dermatitis. Exogenous interleukin (IL)-2 was added to compensate for possible functional differences in accessory cells. Anti-CD3-induced secretion of IL-4, IL-5, IL-6, IL-10, IL-13, and interferon-γ (IFN-γ) was analyzed by enzyme-linked immunosorbent assay (ELISA). Atopy was associated with a low proportion of CD2+ lymphocytes. Responsiveness to PHA, which activates lymphocytes partly via the sheep erythrocyte receptor, CD2, was reduced in the allergic children. The anti-CD3-induced proliferation declined more rapidly with antibody dilution in the allergic than in the non-allergic children. Atopic dermatitis was associated with high levels of anti-CD3-stimulated IL-5 secretion. The IL-4/IL-10 and IL-4/IFN-γ ratios were higher in children with elevated total immunoglobulin E (IgE) levels. Skin prick test-negative children with eczema produced higher levels of IL-10 than skin prick test-positive children. In conclusion, atopic children have a reduced T-cell function. Atopic dermatitis is associated with increased IL-5 production, while high total IgE levels are associated with high IL-4/IFN-γ and IL-4/IL-10 ratios.
Nyckelord
- MEDICINE
- MEDICIN
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- ref (ämneskategori)
- art (ämneskategori)
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