Sökning: WFRF:(Berg Stefan) > Effect of nitric ox...
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000 | 03220naa a2200325 4500 | |
001 | oai:DiVA.org:liu-40472 | |
003 | SwePub | |
008 | 091010s2007 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-404722 URI |
024 | 7 | a https://doi.org/10.1007/s10620-006-9439-z2 DOI |
040 | a (SwePub)liu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Berg, Anna,d 1975-u Linköpings universitet,Cellbiologi,Hälsouniversitetet4 aut0 (Swepub:liu)annbe52 |
245 | 1 0 | a Effect of nitric oxide on histamine-induced cytological transformations in parietal cells in isolated human gastric glands |
264 | c 2006-12-14 | |
264 | 1 | b Springer Science and Business Media LLC,c 2007 |
338 | a print2 rdacarrier | |
520 | a Previous studies have shown that nitric oxide (NO) inhibits histamine-induced gastric acid secretion in isolated human gastric glands. NO synthase has been found to be present in the human oxyntic mucosa and has been suggested to serve as a paracrine regulator of gastric acid secretion. Histamine stimulation of parietal cells induces cytoskeletal rearrangements, recruitment of H +/K +-ATPase-rich tubulovesicles to the apical membrane and expansion of intracellular canaliculi. The aim of the present study was thus to investigate (i) the effect of an NO donor on histamine-induced cytological transformations and (ii) the influence of increased [Ca 2+] i on NO-induced morphological changes in human parietal cells. Human gastric glands were isolated and subjected to the NO donor SNAP prior to histamine administration. [Ca 2+] i was increased by photolysis of the caged Ca 2+ compound NP-EGTA. The distribution of F-actin, ezrin, and H +/K +-ATPase was assessed by confocal microscopy. Ultrastructural analysis was performed using transmission electron microscopy. SNAP did not influence the histamine-induced translocation of F-actin, ezrin, and H +/K +-ATPase but prevented an increase in the canalicular size. Elevation of [Ca 2+] i in resting cells was found to mimic histamine-induced intraparietal cell transformations; however, NO-induced parietal cell morphology was unaffected by a rise in [Ca 2+] i. These results indicate that NO inhibits secretion of fluid into the canalicular lumen without affecting membrane recruitment and that this effect is Ca 2+-insensitive. © 2006 Springer Science+Business Media, Inc. | |
653 | a MEDICINE | |
653 | a MEDICIN | |
700 | 1 | a Redéen, Stefan,d 1961-u Linköpings universitet,Kirurgi,Hälsouniversitetet4 aut0 (Swepub:liu)stere32 |
700 | 1 | a Sjöstrand, Sven-Erik,d 1938-u Linköpings universitet,Farmakologi,Hälsouniversitetet4 aut0 (Swepub:liu)svesj65 |
700 | 1 | a Ericson, Ann-Charlott,d 1965-u Linköpings universitet,Cellbiologi,Hälsouniversitetet4 aut0 (Swepub:liu)anner00 |
710 | 2 | a Linköpings universitetb Cellbiologi4 org |
773 | 0 | t Digestive Diseases and Sciencesd : Springer Science and Business Media LLCg 52:1, s. 126-136q 52:1<126-136x 0163-2116x 1573-2568 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-40472 |
856 | 4 8 | u https://doi.org/10.1007/s10620-006-9439-z |
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