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Abnormal Antinuclea...
Abnormal Antinuclear Antibody Titers Are Less Common Than Generally Assumed in Established Cases of Systemic Lupus Erythematosus
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- Sjöwall, Christopher, 1975- (author)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Länskliniken för Reumatologi i Östergötland
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Sturm, Martin (author)
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- Dahle, Charlotte, 1956- (author)
- Linköpings universitet,Hälsouniversitetet
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Bengtsson, Anders A (author)
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Jönsen, Andreas (author)
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Sturfelt, Gunnar (author)
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- Skogh, Thomas, 1952- (author)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Reumatologi,Länskliniken för Reumatologi i Östergötland
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(creator_code:org_t)
- 2008
- 2008
- English.
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In: Journal of Rheumatology. - 0315-162X .- 1499-2752. ; 35, s. 1994-2000
- Related links:
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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Abstract
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- OBJECTIVE: To evaluate antinuclear antibody (ANA) tests in established cases of systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) by indirect immunofluorescence microscopy (F-ANA) and enzyme-immunoassays detecting antinucleosomal antibodies (ANSA-EIA). METHODS: Sera from 50 patients with SLE and 65 patients with RA were analyzed regarding abnormal concentrations of F-ANA (serum dilution >/= 1:200 = 95th percentile among 300 healthy blood donors). The sera were also analyzed with 2 commercial ANSA-EIA kits. RESULTS: An abnormal F-ANA titer occurred in 76% of the SLE sera compared to 23% in RA, and was not related to present use of antirheumatic drugs. At dilution 1:50, 84% of the SLE sera were F-ANA-positive compared to 20% of healthy women. Forty percent and 56%, respectively, of the SLE sera tested positive in the 2 ANSA-EIA kits. By the most sensitive assay, 96% of the ANSA-positive SLE sera produced a homogenous (chromosomal) F-ANA staining pattern compared to 18% of the ANSA-negative SLE sera. Ten of the 15 F-ANA-positive RA sera (63%) generated homogenous F-ANA staining and 13 (20%) tested positive in the most sensitive ANSA-EIA, but with no correlation to the F-ANA staining pattern. CONCLUSION: The sensitivity of F-ANA at an abnormal titer was surprisingly low (76%) in established cases of SLE. ANSA occurred in 56% of the SLE sera, but also in a fair number (20%) of RA sera. Practically all ANSA-positive SLE sera were identified by chromosomal F-ANA staining. We conclude that the antigen-specific antinucleosomal EIA does not have high enough diagnostic specificity to justify use of this analysis for routine diagnostic purposes.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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