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Site-directed fluor...
Site-directed fluorescence probing to dissect the calcium-dependent association between soluble tissue factor and factor VIIa domains
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- Carlsson, Karin (author)
- Linköpings universitet,Biokemi,Tekniska högskolan
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- Osterlund, Maria (author)
- University Holding, Teknikringen 7, SE-581 83 Linköping, Sweden
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- Persson, Egon (author)
- Vascular Biochemistry, Novo Nordisk A/S, Novo Nordisk Park, DK-2760 Måløv, Denmark
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- Freskgard, P.-O. (author)
- Freskgård, P.-O., Protein Biotechnology, Novo Nordisk A/S, Novo Allé, DK-2880 Bagsværd, Denmark, Molecular Biology, Maxygen ApS, DK-2970, Hørsholm, Denmark
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- Carlsson, Uno (author)
- Linköpings universitet,Tekniska högskolan,Biokemi
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- Svensson, Magdalena (author)
- Linköpings universitet,Tekniska högskolan,Biokemi
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(creator_code:org_t)
- 2003
- 2003
- English.
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In: Biochimica et Biophysica Acta - Proteins and Proteomics. - 1570-9639 .- 1878-1454. ; 1648:1-2, s. 12-16
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- We have used the site-directed labeling approach to study the Ca 2+-dependent docking of factor VIIa (FVIIa) to soluble tissue factor (sTF). Nine Ca2+ binding sites are located in FVIIa and even though their contribution to the overall binding between TF and FVIIa has been thoroughly studied, their importance for local protein-protein interactions within the complex has not been determined. Specifically we have monitored the association of the ?-carboxyglutamic acid (Gla), the first EGF-like (EGF1), and the protease domains (PD) of FVIIa to sTF. Our results revealed that complex formation between sTF and FVIIa during Ca2+ titration is initiated upon Ca2+ binding to EGF1, the domain containing the site of highest Ca2+ affinity. Besides we showed that a Ca 2+-loaded Gla domain is required for an optimal association of all domains of FVIIa to sTF. Ca2+ binding to the PD seems to be of some importance for the docking of this domain to sTF. © 2003 Elsevier Science B.V. All rights reserved.
Keyword
- Ca2+ dependence
- Factor VIIa
- Fluorescence
- Protein-protein interaction
- Tissue factor
- Titration
- TECHNOLOGY
- TEKNIKVETENSKAP
Publication and Content Type
- ref (subject category)
- art (subject category)
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