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Activation of HIV-1...
Activation of HIV-1 specific CD4 and CD8 T cells by human dendritic cells : Roles for cross-presentation and non-infectious HIV-1 virus
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- Larsson, M. (författare)
- Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, NY, United States
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- Fonteneau, J.-F. (författare)
- Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, NY, United States
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- Lirvall, M. (författare)
- Linköpings universitet,Cellbiologi,Hälsouniversitetet
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- Haslett, P. (författare)
- Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, NY, United States
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- Lifson, J.D. (författare)
- Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, NY, United States, Retroviral Pathogenesis Laboratory, AIDS Vaccine Program, National Cancer Institute, Fredrick, MD, United States
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- Bhardwaj, N. (författare)
- Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, NY, United States
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(creator_code:org_t)
- Ovid Technologies (Wolters Kluwer Health), 2002
- 2002
- Engelska.
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Ingår i: AIDS. - : Ovid Technologies (Wolters Kluwer Health). - 0269-9370 .- 1473-5571. ; 16:10, s. 1319-1329
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: The CD4 T cells in mucosal subepithelia are the first cells to become infected during sexual transmission of HIV-1. Dendritic cells (DC) are located in the same area and are known to play a central role in antiviral immune responses. However, extensive viral replication, syncytia formation and cell death follows the interaction between T cells and DC previously exposed to HIV-1. Despite this, anti-HIV responses are generated that control viremia following acute infection.Objective: The anti-HIV-1 cellular immune responses observed may be activated by sources other than productively infected DC. HIV-1 induces apoptosis both in cells it infects and in bystander cells. Furthermore, retroviral replication typically generates a predominance of defective particles. We tested whether DC exposed to antigen from either of these sources could elicit anti-HIV specific immune responses.Design and methods: Apoptotic or necrotic monocytes infected with vaccinia virus vectors encoding HIV antigens, a cell line with integrated HIV-1 and apoptotic CD4 T cells pulsed with non-infectious or infectious HIV-1 virus were used as sources of antigens to assess cross presentation by DC. Furthermore, direct DC presentation of antigen from non-infectious and infectious HIV-1 was examined.Results: We find that dead cells expressing HIV-1 antigens as well as non-infectious HIV-1 particles can be acquired and processed by DC, leading to the activation, differentiation and expansion of viral antigen-specific CD4 and CD8 T cells from seropositive individuals.Conclusions: These sources of antigens may be critical for the generation and maintenance of anti-HIV-1 immunity by DC.
Nyckelord
- Antigen presentation
- Apoptosis
- CD4 T cells
- CD8 T cells
- Dendritic cells
- HIV-1
- Immune response
- NATURAL SCIENCES
- NATURVETENSKAP
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