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Sökning: id:"swepub:oai:DiVA.org:liu-47133" > Distribution and Fu...

  • Streng, T.Department of Pharmacology, Drug Development and Therapeutics, University of Turku, Turku, Finland, Department of Clinical Chemistry and Pharmacology, Lund University Hospital, Lund, Sweden (författare)

Distribution and Function of the Hydrogen Sulfide-Sensitive TRPA1 Ion Channel in Rat Urinary Bladder

  • Artikel/kapitelEngelska2008

Förlag, utgivningsår, omfång ...

  • Elsevier BV,2008
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:liu-47133
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-47133URI
  • https://doi.org/10.1016/j.eururo.2007.10.024DOI
  • https://lup.lub.lu.se/record/1140580URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Objectives: To investigate the distribution of the transient receptor potential (TRP) A1 ion channel in the rat urinary bladder, and to study the effects of hydrogen sulfide (H2S) and known TRPA1 activators on micturition in conscious rats and on heterologously expressed ion channels. Methods: The expression of TRPA1 in urinary bladder was studied with fluorescence immunohistochemistry and real-time PCR in female Sprague-Dawley rats. Cystometric investigations were performed in conscious animals subjected to intravesical administration of sodium hydrogen sulfide (NaHS, donor of H2S), allyl isothiocyanate (AI), and cinnamaldehyde (CA). Fluorometric calcium imaging was used to study the effect of NaHS on human and mouse TRPA1 expressed in CHO cells. Results: TRPA1 immunoreactivity was found on unmyelinated nerve fibres within the urothelium, suburothelial space, and muscle layer as well as around blood vessels throughout the bladder. All TRPA1 immunoreactive nerves fibres also expressed TRPV1 immunoreactivity and vice versa. TRPA1 was also detected in urothelial cells at both transcriptional and protein levels. AI increased micturition frequency and reduced voiding volume. CA and NaHS produced similar changes in urodynamic parameters after disruption of the urothelial barrier with protamine sulfate. NaHS also induced calcium responses in TRPA1-expressing CHO cells, but not in untransfected cells. Conclusions: The expression of TRPA1 on C-fibre bladder afferents and urothelial cells together with the finding that intravesical TRPA1 activators initiate detrusor overactivity indicate that TRPA1 may have a role in sensory transduction in this organ. The study also highlights H2S as a TRPA1 activator potentially involved in inflammatory bladder disease. © 2007.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Högestätt, EdwardLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,Högestätt, E.D., Department of Clinical Chemistry and Pharmacology, Lund University Hospital, Lund, Sweden(Swepub:lu)kfar-eho (författare)
  • Hedlund, PetterLund University,Lunds universitet,Linköpings universitet,Klinisk farmakologi,Hälsouniversitetet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kfar-phe (författare)
  • Andersson, D.A.Wolfson Centre for Age-Related Diseases, King's College London, London, United Kingdom (författare)
  • Jordt, S.-E.Department of Pharmacology, Yale University School of Medicine, New Haven, CT, United States (författare)
  • Bevan, S.Wolfson Centre for Age-Related Diseases, King's College London, London, United Kingdom (författare)
  • Andersson, Karl-ErikLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,Department of Clinical Chemistry and Pharmacology, Lund University Hospital, Lund, Sweden, Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston Salem, NC, United States(Swepub:lu)kfar-kea (författare)
  • Hogestatt, E.D.Högestätt, E.D., Department of Clinical Chemistry and Pharmacology, Lund University Hospital, Lund, Sweden (författare)
  • Zygmunt, PeterLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,Department of Clinical Chemistry and Pharmacology, Lund University Hospital, Lund, Sweden(Swepub:lu)kfar-pzy (författare)
  • Sturesson, HelenaLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)klin-hax (författare)
  • Department of Pharmacology, Drug Development and Therapeutics, University of Turku, Turku, Finland, Department of Clinical Chemistry and Pharmacology, Lund University Hospital, Lund, SwedenAvdelningen för klinisk kemi och farmakologi (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:European Urology: Elsevier BV53:2, s. 391-4000302-28381873-7560

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