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Designed Surface wi...
Designed Surface with Tunable IgG Density as an in Vitro Model for Immune Complex Mediated Stimulation of Leukocytes
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- Kanmert, Daniel (author)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
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- Enocsson, Helena (author)
- Linköpings universitet,Reumatologi,Tekniska fakulteten
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- Wetterö, Jonas (author)
- Linköpings universitet,Reumatologi,Hälsouniversitetet
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- Kastbom, Alf (author)
- Östergötlands Läns Landsting,Linköpings universitet,Reumatologi,Hälsouniversitetet,Länskliniken för Reumatologi i Östergötland
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- Skogh, Thomas (author)
- Östergötlands Läns Landsting,Linköpings universitet,Reumatologi,Hälsouniversitetet,Länskliniken för Reumatologi i Östergötland
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- Enander, Karin (author)
- Linköpings universitet,Molekylär fysik,Tekniska fakulteten
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(creator_code:org_t)
- 2009-10-29
- 2010
- English.
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In: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 26:5, s. 3493-3497
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- We present the design of an in vitro for immune-complex-mediated stimulation of leukocytes and its functional characteristics with respect to monocyte adhesion. The model was based on orientation-controlled immobilization of a humanized IgG1 monoclonal antibody (rituximab) via its interaction with a biotinylated peptide epitope derived from the CD20 marker. The peptide was linked to neutravidin covalently attached to it mixed self-assembled monolayer of carboxyl- and methoxy-terminated oligo(ethylene glycol) alkane thiolates on gold. The surface adhesion propensity of human monocytes (cell line U917) was highly dependent on the lateral IgG density and indicated that there exists a distance between IgG-Fc on the surface where interactions with Fc gamma receptors are optimal. This well-defined platform allows for a careful control of the size and orientation of artificial IgG immune complexes, it is easily made compatible with, for example, cellular imaging, and it will become useful for in vitro studies on the importance of Fc gamma receptor interactions in chronic immune-mediated diseases.
Keyword
- TECHNOLOGY
- TEKNIKVETENSKAP
Publication and Content Type
- ref (subject category)
- art (subject category)
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