Search: id:"swepub:oai:DiVA.org:liu-55024" >
Efficient imaging o...
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Berg, Ina,1982-Linköpings universitet,Biokemi,Tekniska fakulteten
(author)
Efficient imaging of amyloid deposits in Drosophila models of human amyloidoses
- Article/chapterEnglish2010
Publisher, publication year, extent ...
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2010-04-29
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Macmillan Publishers Ltd.2010
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:liu-55024
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-55024URI
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https://doi.org/10.1038/nprot.2010.41DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Drosophila melanogaster is emerging as an important model system for neurodegenerative disease research. In this protocol, we describe an efficient method for imaging amyloid deposits in the Drosophila brain, by the use of a luminescent-conjugated oligothiophene (lco), p-Ftaa polymer probe. We also demonstrate the feasibility of co-staining with antibodies and compare the lco staining with standard amyloid-specific probes. the lco protocol enables high-resolution imaging of several different protein aggregates, such as aβ1-42, aβ1-42e22G, transthyretin V30M and human tau, in the Drosophila brain. aβ and tau aggregates could also be distinguished from each other because of distinct lco emission spectra. Furthermore, this protocol enables threedimensional brain mapping of amyloid distribution in whole-mount Drosophila brains. the use of p-Ftaa combined with other probes, antibodies and/or dyes will aid the rapid characterization of various amyloid deposits in the rapidly growing number of Drosophila models of neurodegenerative diseases.
Subject headings and genre
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NATURAL SCIENCES
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NATURVETENSKAP
Added entries (persons, corporate bodies, meetings, titles ...)
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Nilsson, PeterLinköpings universitet,Organisk Kemi,Tekniska högskolan(Swepub:liu)petni61
(author)
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Thor, StefanLinköpings universitet,Utvecklingsbiologi,Hälsouniversitetet(Swepub:liu)steth80
(author)
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Hammarström, PerLinköpings universitet,Biokemi,Tekniska högskolan(Swepub:liu)perha81
(author)
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Linköpings universitetBiokemi
(creator_code:org_t)
Related titles
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In:Nature Protocols: Macmillan Publishers Ltd.5:5, s. 935-9441754-21891750-2799
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